The Proteomics Core Facility offers support to researchers from the CNIC and other institutions, both public and private. The Unit is aimed at the separation, quantification, identification and characterisation of proteins in biological systems by 2-dimensional gel electrophoresis, multidimensional liquid chromatography, and mass spectrometry (MALDI-TOF and ESI Ion-Trap).
The Unit's biomedical research support is focused on the search for appropriate experimental design, the constant improvement and optimisation of protocols, and the actualisation of equipment and expertise. The laboratory has a comprehensive system for the separation of proteins by 2-dimensional gel electrophoresis, including the necessary technology for Difference Gel Electrophoresis (DIGE: 2-dimensional gel electrophoresis with pre-labelling of samples with differential fluorochromes). As an alternative "gel-free" approach, a complete nano-HPLC system enables multidimensional liquid chromatography analysis. The identification and characterisation of proteins is achieved with MALDI-TOF/TOF and ESI Ion-Trap spectrometers, and a protein picker for the retrieval of proteins from gels and an automatic sample digester aid the automation of the process. For more info please visit the TECHNIQUES SECTION and the EQUIPMENT SECTION.
We are now working with a new triple quadruple linear ion trap mass spectrometer for the improved analysis and characterization of chemical and post-translational modifications in proteins.
Further information about the services offered by this Unit and how to apply can be found on the HOW TO BOOK SECTION
Dr. Jesús Vázquez graduated in Physical Chemistry at the Universidad Complutense (Madrid, 1982) and carried out his PhD in Biochemistry at the Universidad Autónoma (Madrid, 1986), both with Special Distinction. During his postdoctoral training at Merck Sharp Research Laboratories (NJ, USA) and at the Centro de Biología Molecular Severo Ochoa (Madrid), he specialized in protein chemistry and in the study of biomembranes in the context of neurochemical diseases. Since then, he has played a pioneering role in the development of protein chemistry, mass spectrometry and proteomics in Spain. His laboratory has made relevant contributions to the field addressing subjects such as peptide fragmentation mechanisms, de novo peptide sequencing, and analysis of posttranslational modifications. In the last years he has devoted a considerable effort in the development of second generation techniques, relative proteome quantification by stable isotope labeling, advanced algorithms for quantitative data integration and systems biology, and high-throughput characterization of modifications produced by oxidative stress. These techniques have been applied to several research projects, including the study of molecular mechanisms underlying processes such as angiogenesis and nitroxidative stress in endothelium, of ischemia-preconditioning in cardiomyocites and of the interactome at the immune synapse. Author of more than one hundred international publicatons, he is Profesor de Investigación of the CSIC and director of the Proteomics Platform of RECAVA (Spanish Cardiovascular Research Network). He joined the CNIC as a Full Professor in 2011, where he leads the Cardiovascular Proteomics laboratory and is also in charge of the Proteomics Unit.