iSuRe-Cre as a new genetic tool to modify and understand gene function

  • Most analysis of gene function in biomedical research relies on the use of Cre-lox technology. Since its introduction in 1994, this technology has revolutionized biomedical science because it allows scientists to eliminate or activate the function of any gene in any cell type in the mouse. Because the Cre-loxP recombination event is invisible, genetic and fluorescent reporters have been developed to track Cre activity, so that cells reaching a certain threshold of Cre activity are labeled. However, the main problem is that often the Cre activity level is insufficient to fully recombine and eliminate expression of the target gene, generating uncertainty about whether the desired genetic modification has been achieved.

    CNIC researchers have developed a method that increases the efficiency and reliability of the analysis of Cre-dependent gene function. Moreover, the use of this genetic tool permits the induction of multiple genetic deletions in the same cell, to allow the study of functional genetic interactions.

    Research article

    Number
    PCT/EP2020/051407
    Priority date
    22/01/2019
    Applicants
    CNIC
    Inventors
    Rui Benedito, Macarena Fernández