Junior Postdoctoral Position in Metabolomics

Estado: 
Cerrada
Fin del plazo de presentación de solicitudes: 
Miércoles, Junio 14, 2017

 Junior Postdoctoral Position in Metabolomics available at Spanish Cardiovascular Research Centre (CNIC) Madrid (Spain)

A junior postdoctoral position is available to develop a joint project of the laboratories directed by Dr. David Sancho, Dr. Jesús Mª Vázquez and Dr. Borja Ibáñez at the CNIC.

The project will investigate the gut microbiome and its related metabolites in the progression of the atherosclerosis. We are looking for an enthusiastic and highly-motivated person with an interest in analytical sciences focused on Metabolomics.

Funding is available from summer 2017. In addition the selected candidate will apply for a Juan de la Cierva Fellowship in the next call.

 For more information about the laboratories see:

https://www.cnic.es/en/investigacion/immunobiology

https://www.cnic.es/en/investigacion/cardiovascular-proteomics

https://www.cnic.es/en/investigacion/translational-laboratory-cardiovascular-imaging-and-therapy

 Job Description:

  • Conduct metabolomics/lipidomics studies with emphasis on methodologies and expertise related to coupled high performance liquid chromatography and mass spectrometry
  • Develop and optimize methods to simultaneously measure large numbers of analytes in biological samples
  • Work as a key member and contributor to a larger team in a translational project
  • Contribute to experimental research design and planning
  • Manage project efficiently to meet timelines

Minimum requirements:

  • PhD awarded after 1st January 2016 in chemistry or biochemistry or equivalent life science
  • Applicant must have at least one paper published as first author in high quality journal

Assessable requirements:

  • Expertise in targeted and untargeted metabolomics employing HPLC coupled to mass spectrometry, including sample preparation, analysis optimization and method development is required. Expertise in shotgun lipidomics or in mass spectrometry-based proteomics will also be considered
  • Expertise in data treatment for targeted and untargeted metabolomics will be valued
  • Able to communicate and collaborate effectively to deliver on-time and high quality results
  • Demonstrated ability to work effectively both independently and as part of a cross-functional team
  • Ability to develop a positive working relationship with a wide range of colleagues inside and outside of the CNIC
  • Stays abroad will be acknowledged 

SELECTED RECENT PAPER OF THE GROUPS:

Inmunobiology lab, directed by Dr. David Sancho:

.- Iborra S et al. Optimal Generation of Tissue-Resident but Not Circulating Memory T Cells during Viral Infection Requires Crosspriming by DNGR-1+ Dendritic Cells. Immunity 2016; 45(4): 847-860 (2016).

 .-Iborra S et al. Leishmania Uses Mincle to Target an Inhibitory ITAM Signaling Pathway in Dendritic Cells that Dampens Adaptive Immunity to Infection. Immunity 2016; 45(4):788-801.

 .- Garaude J et al. Mitochondrial respiratory-chain adaptations in macrophages contribute to

antibacterial host defense. Nat. Immunol 2016; 17(9): 1037-45.

 .-Sánchez-Paulete AR et al. Cancer immunotherapy with immunomodulatory anti-CD137 and anti-PD-1 monoclonal antibodies requires Batf3-dependent dendritic cells. Cancer Discov 2016; 6(1):71-9.

 .- Blanco-Menéndez N et al. SHIP-1 Couples to the Dectin-1 hemITAM and Selectively Modulates Reactive Oxygen Species Production in Dendritic Cells in Response to Candida albicans. J. Immunol 2015; 195(9): 4466-78.

 .-Martínez-López M et al. Batf3-dependent CD103+ dendritic cells are major producers of IL-12 that drive local Th1 immunity against Leishmania major infection in mice. Eur. J. Immunol 2015; 45:119-29.

 .- Iborra S et al. Signaling versatility following self and non-self sensing by myeloid C-type lectin receptors. Immunobiology 2015; 220:175-84.

 .- Iborra S et al. The DC receptor DNGR-1 mediates cross-priming of CTLs during vaccinia virus infection in mice. J. Clin. Invest 2012; 122(5):1628 - 1643.

 .- Sancho D et al. Signaling by myeloid C-type lectin receptors in immunity and homeostasis. Ann. Rev. Immunol 2012; 30: 491-529.

  Cardiovascular Proteomics lab, directed by Dr. Jesús Mª Vázquez:

.- Bartolomé-Izquierdo N et al. miR-28 regulates the germinal center reaction and blocks tumor growth in preclinical models of non-Hodgkin lymphoma. Blood  2017;129(17): 2408-2419.

.- Burillo E et al. Quantitative HDL Proteomics Identifies Peroxiredoxin-6 as a Biomarker of Human Abdominal Aortic Aneurysm. Sci Rep 2016; 6: 38477.

.- Cogliati S et al. 2016. Mechanism of super-assembly of respiratory complexes III and IV. Nature 2016; 539(7630): 579-582.

.- Cibrián D et al. CD69 controls LAT1/CD98-dependent L-tryptophan uptake and AHR-mediated IL-22 secretion in psoriasis. Nat Immunol 2016; 17(8):985-996.

.-. Latorre-Pellicer A et al. Mitochondrial and nuclear DNA matching shapes metabolism and healthy ageing. Nature 2016; 535(7613):561-565.

- García-Marqués F et al. A novel systems-biology algorithm for the analysis of coordinated protein responses using quantitative proteomics. Mol Cell Proteomics 2016; 15(5): 1740-60.

.- Osorio, FG et al. Loss of the proteostasis factor AIRAPL causes myeloid transformation by deregulating IGF-1 signaling. Nat Med 2016;22(1):91-96.

.-. Gonzalez-Teran, B et al. p38 gamma and delta promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation. Nat Commun 2016; 7;10477.

.- Bonzon-Kulichenko E et al. Revisiting Peptide Identification by High-Accuracy Mass Spectrometry: Problems Associated with the Use of Narrow Mass Precursor Windows. J Proteome Res 2015; 14(2):700-710.

.- Navarro P et al. General Statistical Framework for Quantitative Proteomics by Stable Isotope Labeling. J Proteome Res 2014; 13(3):1234-1247.

Translational Laboratory for Cardiovascular Imaging and Therapy lab, directed by Dr. Borja Ibañez.

.-Wai T et al. Imbalanced OPA1 processing and mitochondrial fragmentation causes heart failure in mice. Science 2015;350(6265): aad0116 (pages 1-11).

.- Fernández-Jiménez R et al. Myocardial Edema after Ischemia/Reperfusion is not stable and follows a bimodal pattern: advanced imaging and histological tissue characterization. J Am Coll Cardiol 2015;65:315-23.

 .- Ibanez B et al. Effect of early metoprolol on infarct size in ST-segment elevation myocardial infarction patients undergoing primary PCI: the METOCARD-CNIC trial. Circulation 2013; 128:1495-1503.

.- García-Álvarez A et al. Noninvasive Monitoring of Serial Changes in Pulmonary Vascular Resistance and Acute Vasodilator Testing using Cardiac Magnetic Resonance. J Am Coll Cardiol 2013;62:1621-31.

 .- Ibanez B et al. Evolving therapies for myocardial ischemia/reperfusion injury. J Am Coll Cardiol 2015;65:1454-71. (Review).  

 .- Pizarro G et al. Long term benefit of early pre-reperfusion metoprolol administration in patients with acute myocardial infarction: results from the METOCARD-CNIC trial. J Am Coll Cardiol. 2014;63:2356-62.          

 .- Fernández-Friera L et al. Prevalence, Vascular Distribution and Multi-territorial Extent of Subclinical Atherosclerosis in a Middle-Aged Cohort: The PESA (Progression of Early Subclinical Atherosclerosis) Study. Circulation 2015;131:2104-2113.

 - García-Ruiz JM et al. Impact of the timing of metoprolol administration during ongoing STEMI on infarct size and ventricular function. J Am Coll Cardiol 2016;67:2093-104.        

 - Fernández-Jiménez R et al. Pathophysiology Underlying the Bimodal Edema Phenomenon after Myocardial Ischemia/Reperfusion.  J Am Coll Cardiol 2015; 66:816-28.                     

  Cruz FM et al.  Exercise triggers arrhythmogenic right ventricular cardiomyopathy phenotype in mice expressing a disease-causing mutated version of human plakophilin-2 after single adeno-associated virus-mediated gene transfer. J Am Coll Cardiol 2015;65:1438-50.

The CNIC offers:

  • Incorporation into a consolidated Research Center of international scientific relevance, within the public sector and managed by a Foundation.
  • Integration into an excellent scientific environment.
  • Access to a modern infrastructure and advanced technologies.
  • Significant possibilities for personal development.
  • A competitive salary corresponding to a junior postdoctoral scale at CNIC, equivalent to Sara Borrell or Juan de la Cierva.
  • Facilities for specific training in novel aspects related with the offered job.

 

SELECTION PLAN: The candidates with the highest score (if greater than 70 points) will be interviewed and up to two candidates with the highest score will be hired (if greater than 90 points).

The CNIC is a center that advocates equal job opportunities for men and women, foreign and national, and promote nondiscrimination by reason of age, race or ethnic origin, religion or belief, sexual orientation, language, disability, political orientation or social or economic conditions. The CNIC ensures the utmost rigor in the application of the principles of equality, merit and ability in public employment and current rules on data protection.

Criterios de puntuación: 
C1 - Experience in targeted and untargeted metabolomics - 15%
C2 - Experience in the following techniques (HPLC or UHPLC coupled to mass spectrometry, including sample preparation, analysis optimization and method; Experience in lipidomics analysis (including shotgun lipidomics), lipid extraction methods or MS-based prot - 55%
C3 - Fluent English - 10%
C4 - Interview (only those candidates with more than 70 points in the sum of the scores of the previous requirements will be interviewed) - 20%

"En caso de ausencia de alguno de los evaluadores se nombrará un evaluador alternativo de la misma área"