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{"sheet":"CD4 Full","index":0,"n_rows":32,"n_cols":29,"rows_kept":32,"cut":false,"hidden":true,"merged":[]}
["Column1","Column2","Column3","Column4","Column52","Column5","Column6","Column29","Column7","Column8","Column9","Column10","Column11","Column12","Column13","Column14","Column15","Column16","Column17","Column18","Column19","Column20","Column21","Column22","Column23","Column25","Column26","Column27","Column28"]
["SSM","APC-A","APC-Cy7-A","APC-R700-A","BB515-A","BB630-A","BB660-A","BB700-A","BB755-A","BB790-A","BUV395-A","BUV496-A","BUV563-A","BUV615-A","BUV661-A","BUV737-A","BUV805-A","BV421-A","BV480-A","BV570-A","BV605-A","BV650-A","BV711-A","BV750-A","BV786-A","PE-A","PE-CF594-A","PE-Cy5-A","PE-Cy7-A"]
["APC-A",0,2.1522083913314032,3.7779094112614691,0,0.26367304534774444,1.7008190483364984,0.51281910511040385,0.5263493801760466,0.20416508204204248,3.9220474543458879E-3,0,0,9.6584024299340029E-2,1.1344375040946975,1.1372367528696243,0.83103262623722962,0.16984375285583553,0,0,0,0.70711030923094209,0.75638962874988858,0.64613274621252292,0,0,0.20304186175980743,1.98747392936457,1.7604697141661934]
["APC-Cy7-A",0.501331435662979,0,1.0572957455572298,0,0.18082458965884604,0.31826276544671578,0.16789437966336168,0.65409444652766469,0.72993855631605797,0.23307104477048501,0,0,9.4216432573209108E-2,0.16577940813682576,0.47175107607847666,2.1597770049539138,0.11653615685201624,0,0,0,0.25181051908562302,0.27350234264111273,0.56687540403673931,0,0,0,0.27239800489662863,3.7114435905260401]
["APC-R700-A",0.77703862547079972,2.644736257748272,0,0,0,0.42908563301866326,0.60863311829646127,0.82502927434017703,0.41537305044280576,0.11624545012265255,0,0,0,0.26363014116402789,1.4996048930567469,0.96538115394797941,9.4742630700392447E-2,0,0,6.5589419666242557E-2,0.20522913318468791,0.64679194075575663,0.60843159925924606,0.20425767147767851,0,0.11311371473330187,0.37516592878408178,2.0885346863328587]
["BB515-A",0.32687486802969407,0.21799027762170467,0.12651378485425818,0,0.93787961575253675,0.67346643731381683,0.49796787184538777,0.51489256527854377,0.12776710973397848,0.11235848184055897,0.15955169804661784,0.11273784698751024,7.4508436437448644E-2,0.18670273724864542,0.16920824536350773,0,0,0.10371734398071968,6.9016003623682759E-3,0.12892150735349678,0.22159144655182492,0.21346465244174193,0,0,0,0.29670605850605147,0.14805621166595853,0.21974558430268962]
["BB630-A",1.3516536771850733,0.14937394386577885,0.63033125260382072,0,0,2.315289985742794,2.1241225752987098,2.0759961127251998,0.79514525447087381,0.14777505398646154,0,0.18956938366995035,0.92931817398268646,0.79527500163302345,0.76991669104171978,0.51331967734061712,0.2987363966930926,4.0147886983840003E-2,0.99625855888140646,1.9512961525800161,1.0726126406433858,0.96569505779989662,0.73141189380510885,7.7349085488801594E-2,0.97972921395409962,3.080964618408681,0.98160881148979173,0.7916540666350329]
["BB660-A",4.2820365371990858,1.646611533079253,3.2965650337509911,5.1067770924878166E-2,0.59155647797372735,0,3.645288384642797,3.4566178096065778,1.4036563588530202,0.18101223057682606,0,8.7920570330949091E-2,0.11850274988600058,1.4540390066447524,1.2491644145154606,1.0615377029159636,0.41260021138951952,2.6413475861010375E-3,0.24382503971038363,0.4152023298554674,1.7602192499855063,1.7923475754454556,1.2584655387717925,0.21970969252195457,0,0.30720317116091622,1.4289689596080022,0.82320618944325885]
["BB700-A",1.8234841039890348,1.8521722237999216,3.733954524149258,0,0.56244934352312892,2.6145859282998294,0,5.7402292803419721,1.8660251054547987,0.20428852828548105,0,8.7663082434950648E-2,0.13307024322337527,1.0259777604182982,1.8450279220672621,1.6067055966322756,0.4122808562827634,0,0.21829651917310747,0.244192081336709,1.2075998499366887,2.5695887111808968,1.8592271638353088,0.34321986617615546,0,0.21348729290104873,0.58081702226516319,1.23882904506158]
["BB755-A",0.440515360987211,1.3695669709392804,1.208591328363751,6.9171186770948787E-2,0.6667631641328291,0.93255591294960305,1.9725015458543187,0,2.996495731562665,0.26110212395283378,0,7.2732241806434342E-3,0.11178043021483403,0.25295125003930846,1.6764535335353601,2.0690405055154768,0.2478742169182104,6.1965143696771779E-2,0.16639498169123854,9.5648608226663115E-2,0.41988671143887274,0.78499703019532197,2.0273783458172931,0.56453665352031146,0,0.43387090433390901,3.1167771007418617E-3,1.0335964338843147]
["BB790-A",0.29023351683505305,2.2916624927658305,0.53876773254137489,0,0.5303490329024465,0.73297834707036891,1.3341038355817041,6.5243693132536551,0,0.51956413345724195,0,0,9.8285135153594633E-2,0.20702255092444849,1.0346284683707347,5.081443226061201,0.51831210075036427,5.4555637663121569E-2,0,8.4495065533710612E-2,0.31174230956395627,0.43016024626482291,2.0997077945099685,1.1982709181538787,0,0.31409807160213021,0,1.2597851334342254]
["BUV395-A",9.4272367146311514E-2,0.30686183113125676,9.8112460509560864E-2,0,0.22360570641266941,0,0.14551306229773933,0.22305976699674154,0,0,0,0,0.11676117318652522,0.18296231514841307,0,0.42349890578207294,0.11666761870011445,0,0,9.9980573055787975E-2,0,0.18068909030465338,0,0.12448277533825605,0,0.12144479796339019,0,9.7203732267954465E-2]
["BUV496-A",0.56552976852385251,0,0,8.1699001343754317E-3,0.66197422530828776,0.59688733367113567,0,0.6530072661489168,0,2.8844531167787824,0,0.93358570254391882,0.53738346980129426,0.95741907983480012,0.94125817240133758,0.49331250120910297,0.19574455971105797,0.62099032827106593,0.33790788284247553,0.23816973716137421,0.25745318529110939,0.56110660759726627,0,0.29783095614052268,0.6759080708786579,1.5458472919856685,0.19108658498213693,0.40245699884507441]
["BUV563-A",0.92553436484240892,0,0.22645228180111904,9.5144839872579504E-2,1.8776955826901518,1.02207360134638,0.59318930238284895,0.72969261675999419,0.22812894697099539,1.2811319539688628,0,0,1.0553388597895159,1.4290535426878126,1.1628886365599376,1.1281675823960469,0.24871422776544283,0,0.55699695898803758,0.56028953745920052,0.46369874782196319,0.44719538682830728,2.8228183728317872E-2,0,4.4971454622329503,3.8505938090690064,0.86503553012232193,0.81825866525979096]
["BUV615-A",2.1110670453226241,0.4687171196768507,0.94668915147147936,0,4.9272958506807187,2.0495117316766995,1.0597053759201238,0.96496850513156218,0.48732999560195939,1.0675284524516306,0,0.66555127538586101,0,2.9055638334367329,3.4088651305131354,2.3338562727188243,0.15904129315114968,0,0.53372802210131798,1.5830436153085672,0.89933483171785111,0.67320066010525359,0.51446118840438071,0,2.532714288694232,11.255349217214166,2.011302317780681,2.4189861236295953]
["BUV661-A",6.5661507513458384,1.8441385219570381,3.7213797068266317,0,0.23550951144708374,1.4263441807026964,0.42217467211340265,0.81611350941632588,0.32123267216267831,0.95906198712553858,0,0.1437393103578995,0.10943970242296044,0,3.4134866581917107,2.4395324312785047,0.13526935929476813,0,0,0.13311340784930298,0.74816586424982645,0.79041416581049173,0.58887561978051106,0.23611834432558249,8.7177440693264399E-3,0.47520942134012106,1.5268621076758282,1.4629749277169684]
["BUV737-A",0.32451508393528095,2.2561291588158117,4.9139251704388833,0,0.14598639223246671,0.35619381623966395,0.94157152384680232,2.799698321730014,1.050745704891292,0.72137764964100115,0,0,0,0.25842197214661333,0,3.7541198716116972,0,0,0,0,0.20146375178902215,0.4720205832091886,0.79491139254634713,0,0,0.12922773621952949,8.5417380319320876E-2,1.4880665700055604]
["BUV805-A",0.11949960769389205,1.5889846134118872,5.0609707874187315E-3,0,9.7563384971079387E-2,0.14296165399282224,0,0.38297665041704282,0.36189107242886398,0.97107179593464987,0,0,0.12803228741305892,8.0438673272771127E-2,0.36347659890745054,0,0.12827046772796533,0,0,0,0,0.24958108561366979,0.31727682805335322,0,0,0.21812381973698011,7.1323974394808212E-2,0.74403757532806447]
["BV421-A",0.10333248213998053,8.1066452665959965E-2,7.5181997317487134E-2,0,0.12141620453852353,0.10046716044543375,9.6600492237923552E-2,8.4715068487294556E-2,0.13351373921213217,8.2157515326023223E-2,0.10634710195174711,0,7.7542264491894386E-2,0.1324704350401632,0.16359874706181327,0,0,0.16550314771072164,0.10841060818159053,0,0.11751469903278175,0.19231594374177918,0.17877696180460753,0,5.2422304270099709E-2,0,0,0.11851511982594409]
["BV480-A",0.24113343446029611,0.26923468684387081,0,0,0.52638099615817724,0.27206198577641466,0.34053167190785283,0.36419358927530876,0.25225758914879692,1.2703416052273566,1.2096944321657326,0.61042021290412174,0.39948395744713583,0.54560984491935716,0.57589277630258529,0.91859675420352371,0.54335671868910285,0,1.6576848443898871,1.1344949942897526,0.74954489787900225,0.70463289316589539,0.48430023051253079,0.41261909484121878,0.66650813833717493,0.60499124573603102,0.11691307142523391,0.32085491846078124]
["BV570-A",0.60504761609096414,0.41534795442263117,0.4765087164639778,0,1.8053540118032887,1.245876833603998,0.78438210507833583,1.0456385970197037,0.38923834584641848,5.4420432868285164E-3,0,1.4969041735044359,0.72036792609576028,1.228231837467336,1.06491276573042,1.1924798695386667,1.5190606505364044,0,0,1.9689040898356858,1.7924851861599258,1.6368014936835562,0.48860181840614592,0,3.3281982631883005,3.3971466840839648,1.044803319007626,1.1565489763026884]
["BV605-A",1.2562026120063063,0.49865419992748827,1.0639496676539588,0,2.4549331062359596,1.8320270627874569,0.9077898202732112,1.3448636359711297,0.57720636126630998,0.34996117980054647,0,0.84612381152310445,1.9896493925269696,2.0671317592729452,2.3305464643986835,2.0279434867998782,1.1827410850646691,0,2.164591570895571,0,2.7518768784181677,2.3740998860770688,1.7335472365942912,0.17141643726265479,2.9186730520462048,5.3130482285251475,1.8096326206799176,2.0204610206057714]
["BV650-A",2.6594368388537077,1.0870955852973794,2.1924632672419047,0,0.35100054095612665,1.1745921777033224,0.49677728446873509,0.75469739781933198,0.31613912316782777,0.35833506086658135,0,0,0.36227808362062042,3.0440383767003643,3.0222474003997104,2.4261937316930378,1.053377005067913,0.1294152647890402,0.65504921496123403,0.92445094668349592,0,3.4847911247064514,2.1752949165543662,0,0.23817128548801381,0.92949452817942069,1.1215624578593373,1.1697956253737418]
["BV711-A",0.54759588159200412,2.0020861032987227,4.972228873997226,0,0,0.44591347661902386,1.1779866914104464,1.8910240291314409,0.64649780798671619,0.31984061492280286,0,7.4502701649428946E-2,0,0.56670555803813216,4.9593005777747061,3.1463930693902378,0.62119355531168774,0,0,1.075668930002103E-2,0.84939124541216648,0,2.8885554686834731,0.74208251577370532,0,0.1797997640238265,0.15599282062836231,1.245141413023966]
["BV750-A",0,1.9585158172707258,1.9634192460015119,0,0.18129315968712212,0.21591275151074713,0.6658877857791331,2.4409504020378923,1.0590417891058461,0.53991288914224167,0,0,0.1350944299278177,0.14851286877975695,4.1366229530181364,4.8017090451965361,0.58392000271470956,0,0,0,0.25227801782985038,1.0656149235524561,0,1.2967588696110339,0,0,9.2698845209998104E-2,1.1640167806951365]
["BV786-A",0.17734066968733098,3.5128824238618552,1.087924522358602,0,0.29325634441908588,0.24533242163068605,0.43813136781903966,1.9321748027881778,1.4070018694340694,1.3019146518606115,0,0.16411244891593119,0,0.24118509450819223,3.0688167701607068,11.423426558401042,1.335393404490258,0,0,0,0.40976537508294902,0.76478292191589003,4.8357473894929948,0,0.18292157963292585,0.51632809254020817,0.2139877232625457,2.0871178087967288]
["PE-A",0.65111717378518918,0.16851689197069597,0.26116628302151412,0,2.1888038487672112,1.3508413263122605,0.98978753993924917,0.99720653376043544,0.32610493826604264,0.16689893545724857,0,0.88146364557877266,0.30431262819767751,0.37881536743600469,0.40208652239205217,0.29888222764105055,0.33768735839504116,0,0.9668057043318643,0.76944878477194789,0.42021239215258965,0.45016985764848355,0.2501782304460401,0.12458872156603948,0,3.5299285048713371,0.93499597940491841,0.8286109145231747]
["PE-CF594-A",1.0111085258839672,0.16251611418103623,0.44041125591332464,0,3.9345922085361713,2.237032463585154,1.8638257290175155,1.88528997939919,0.72973812446082076,6.547265235090477E-2,0,9.4025496850627846E-2,0.45622525044999174,0.50946452900548544,0.60165496222849468,0.46088525914888284,0.18967013007994799,0,0.41104414835773939,1.0338439822311281,0.62878378370052068,0.62220611383592317,0.47656952398074309,0.16456636187692367,1.6047133248607299,0,1.6189958042270347,1.6658456973244333]
["PE-Cy5-A",4.5868990097227096,1.2556624023742622,2.7845707859958995,0,0.35003635708544972,5.347062159364846,4.3476937326522007,3.761390039883266,1.4782972109007502,0.16344677562227891,0,4.0936050320368109E-2,6.7444409516486767E-2,1.5491013734516146,1.0504233151556908,0.7344821675598584,0.3136180730978132,0,0.20508599440394432,0.24503948484799284,1.2682987394415242,1.289415257298633,0.85396161291123585,0.20907163061581424,0.42228979529425742,0.41082232096494714,0,3.5962578830572633]
["PE-Cy7-A",9.5846060261822352E-2,0.94473979508963624,0.18594359014463227,0,0.24204886446047449,0.18775520693635966,0.48911288028361666,3.1563216651354695,2.5345108177723827,0.12845913426527425,0,5.5234476389251802E-2,6.4006134961261466E-2,0,0.35855334781992493,1.3399508942451472,0.12886005634109013,0,6.2815218294765038E-2,0,6.785072021944584E-2,0.16632653354420984,0.64910136330768786,0.3768449911483393,0.28720433633597403,0.2884326275186368,7.2556650011138354E-2,0]
[]
["Spread Matrix based on undiluted CD4 single color stains"]

{"sheet":"CD4 1_100","index":1,"n_rows":32,"n_cols":29,"rows_kept":32,"cut":false,"hidden":true,"merged":[]}
["Column1","Column2","Column3","Column4","Column24","Column5","Column6","Column29","Column7","Column8","Column9","Column10","Column11","Column12","Column13","Column14","Column15","Column16","Column17","Column18","Column19","Column20","Column21","Column22","Column23","Column25","Column26","Column27","Column28"]
["SSM","APC-A","APC-Cy7-A","APC-R700-A","BB515-A","BB630-A","BB660-A","BB700-A","BB755-A","BB790-A","BUV395-A","BUV496-A","BUV563-A","BUV615-A","BUV661-A","BUV737-A","BUV805-A","BV421-A","BV510-A","BV480-A","BV605-A","BV650-A","BV711-A","BV750-A","BV786-A","PE-A","PE-CF594-A","PE-Cy5-A","PE-Cy7-A"]
["APC-A",0,2.0270912588431904,3.5589520889912554,0,0,1.8913092440288712,0.42743620063487137,0.65786979043576266,0.34303006865877383,2.2581912814650541E-3,0,0,0,1.1372225196743271,0.9948844019814812,0.60798681696546109,0,0,0,0,0.76524279181761778,0.49032835772453487,0.3296703813127595,0,0,0.26066084746681506,2.0205263785062786,1.8638032409314615]
["APC-Cy7-A",0.75303763280814096,0,1.0000208759107678,0,0,0.41386632340239171,0.16941199583154593,0.49038961831151234,0.64062874949078441,3.2447489562018325E-3,0,0,0,0.20771383876955435,0.54938681376396892,2.0649477737332975,0,0,0,0,0.35869091581079909,0.3458666321094635,0.59036004970700662,0,0,0.19816904199774801,0.23269734192104274,3.3185744166717739]
["APC-R700-A",0.69314267725140721,2.4091974547805033,0,0,0,0.3985699757222001,0.48267022055439407,0.77208308671843118,0.37933709849163105,0,0,0,0,0.27644917537313085,1.2526244291289683,0.83235872630182528,0,0,0,0,0.13466759339919682,0.59955298197664508,0.49914348262484731,0,0,0,0.3527463534952418,1.9519543551792025]
["BB515-A",0.39003622299048368,0.37221768099400343,0,0,0.97038393225434028,0.68794845004138605,0.56953359029251405,0.45473368186261737,0,0.17681529646135666,0,0,0.16870041168374009,0.25729460814045096,0.26566416075640475,0,0.24296627567184514,0,0,0,0.31269414664846806,0.42838927067285226,0,0,0,0.24512402054174512,0,0.19484979841380418]
["BB630-A",1.1694789225453794,0.18655890649043899,0.56486089656560778,0,0,2.4246614605223216,2.0374747229818828,1.9722962615977797,0.70481301052934431,0.12590107300749503,0,0.20593764139125348,0.91989436476513009,0.80986436803039918,0.79502519308523223,0.53427337516817541,0.35738008703874496,0,0.99945002995640608,1.9604312158262134,1.2518084658833777,1.1234688694820425,0.65860942370967579,0,0.95604726285751884,3.4248440063218379,0.9533615800229478,0.89309039643111265]
["BB660-A",3.3599309908233361,1.5774654435111599,2.72581718261885,0,0.51086360809368747,0,3.4068335628435613,3.6132176125981199,1.3656939265423458,9.3094760525661457E-2,0,0,0.15576173979430163,1.407549022660818,1.3656736613670188,1.1326591187494575,0.36307866639897246,0,0.24582519499328645,0.33940210969289136,1.5736937430041154,1.6620136861822732,1.0534779472694229,0,0,0.3475833937569679,1.253416083925319,0.85254935518127684]
["BB700-A",1.4558484328380803,1.7589052480930822,3.7005421077614438,0,0.61121803476655967,2.5826699111351239,0,5.1072536095001944,2.0353952633918539,0.20099479861892147,0,0,9.9821744889608627E-2,0.93532541900293786,1.8380284489864049,1.5012475726877652,0.36405029616625761,0,0.17338542999940351,0.26800811481690967,1.2369137402381287,2.2698582005058277,1.6707556267629655,0.28757120957420412,0,0.33099575669370157,0.56777876746116429,1.1060956727946576]
["BB755-A",0.28795279104681915,1.2757471903044206,0.95164871891583158,0,0.44138519533750864,0.82955934597631864,1.8135099250819779,0,2.6436310983794824,2.9710233624048741E-3,0,0,0.12459321833006805,0.23376710809937928,1.6283379879064352,1.9792978879740797,0.2574377564760969,0,0,0.21772641911887161,0.3289727577653353,0.7705735793402787,2.1326478556494504,0,0,0.2569889156214441,4.9676489287923961E-3,0.95830549982103175]
["BB790-A",0.26041225276571583,1.8079576692241697,0.43387046204361956,0,0.39927301907689061,0.59595842411532851,1.2489029102109372,6.0828258641334223,0,0.47279541735761449,0,0,0.16030090729899032,0.30363535168482564,1.0210042527473313,4.5061550342725649,0.4220107768839223,0,0,9.7660694674925833E-3,0.45346187545913957,0.46283989373094736,2.1255024281162886,1.1050511902222524,0,0,0,1.4275867214745617]
["BUV395-A",0.32059818461559492,0.35666352147897262,0,0,0,0.31506815391452786,0.35161230431377655,3.4769916618162457E-2,0.33667690600055716,0,0,0,0,2.4048759255199174E-2,0.5455857798905257,0,0,0,0,0,0.64290447264499839,0.71610320995476495,0,0,0,0.71471048648710389,0,0.46311486401897911]
["BUV496-A",0.57598552638849621,1.0194957468693702,0,0,7.1183061706312972E-2,0.39699080134994214,0.63004448247062628,0.82724413531987295,0,2.3498152690979777,0,0,0.62376380116811148,1.3289829394896182,0.98743843362223349,1.2865014918003412,0,0.48926830144996808,0,0,0.81014889092465547,0,0,0,0,1.490111980010022,0,0.71738539345089347]
["BUV563-A",0.7491485465438521,0.33334295599660158,0,0,1.6533479790141696,0.85734205649592998,0.46477741577497289,0.9443204090798164,0,1.0084988872191241,0,0,1.0578590399395846,1.338478264181939,1.3321841541319137,1.0605754724244558,0,0,0,0,0.69129980859909645,3.0466259336318292E-2,0,0,3.7381553951638291,3.8170207734252211,0.85441329377222897,0.9947713141129727]
["BUV615-A",2.0373695030623979,0.55824498988733218,0.97563438652276824,0,3.6389541598576782,2.1446918534114929,0.91871320811758206,1.2668052005898915,0.75630812628639077,0.88099759666677768,0,0.53963900610399573,0,2.9129510113035249,3.0350055037488497,1.992264897880633,0,0,0,0.96708281653323269,0.96034307655670015,1.1477269017468088,0.76657557534813359,0,2.2010609110534038,7.1733891327987571,2.114030732191778,2.3636284338900548]
["BUV661-A",4.2626002767553599,1.9205054699728556,3.0719235687240039,0,3.7438956570493856E-2,1.2477761031252061,0.33162489028661951,0.72894530985480555,0.18179726698052173,0.83176368474155016,0,0.22748612838412549,0.26685560767487188,0,3.609513360449137,2.5793773410497329,0.15490840059933175,0,0,0,0.77751488647459654,0.65818389000242405,0.78028133127322163,0,0,0.67910782720566132,1.2315928503627434,1.3810682730684771]
["BUV737-A",0.24280249708033397,2.1634525371931805,4.0772549335968797,0,0,0.23846513210860576,1.0382485925489524,2.4005500552317667,0.89077601511861226,0.57445580733451285,0,0,0.1050890329502312,0.22914957107701314,0,4.0284203842135904,0,0,0,0,0.13641804566051288,0.38306770651806582,0.87864602610673082,0,0,0.15280211061224006,0,1.0229284408347918]
["BUV805-A",0,1.1770752076062501,0.36692293575461382,0,0.33299721604958393,0,0.2239890166068054,0.59560579037967243,0.43334672392534357,0.89841321836729338,0,0,0.17888065868082303,0.33701472798002047,0.28219135890407931,0,0,0,0,0,0,0,0,0,0,2.4451077294940674E-2,0,0.8830243455040413]
["BV421-A",0,0.30176319475338886,0,0,0.31345861121011287,0.21737112161502561,0,0.26042631773778147,0,0,0,0,0.19460859824477422,0.20871133689660146,0.30731536534898213,0,0,0,0,0,0.31065059876156514,0.34730245637739271,0,0,0,0,0,0.22398461488080776]
["BV480-A",0,2.6001708164212815E-2,0,0.18652305033957406,0,1.500009512580563E-2,0,0,0.25653074263745773,0.56728224147891571,0.22631211123259287,0.51716474609681407,0.43819320580179705,0.67666490216350117,0.6485546463946803,0,0.44550755942929043,0,1.2966916308414138,0.77244578127395824,0.91730578068755675,0.67367092139759521,0.99271236302651344,0,0.6776396870666993,0.70747117140829252,0,0.56474431619891552]
["BV570-A",1.0909770594704686,1.0757666717466214,0,0,1.600073732841008,1.2796901667253957,0,0.56128254809868616,0,7.5153780272472492E-3,0,0.89750245710073084,0.73762015039193674,1.4945046146073226,1.2683098826551866,0,1.2563016016904007,0,0,1.296204541235167,1.8865415887529535,1.1996461798923952,1.2725108479058866,0,3.5389715137213638,3.6949380464305901,1.1272467474255272,1.318445661527744]
["BV605-A",1.2881953819207859,1.2951669389008171,0.52904365533457653,0,2.5820782950600516,1.7516140661533377,0.80054618301895453,1.0497572814043696,0,8.0566312416658105E-3,0,0.82937784203185883,1.0324213460903298,1.8534921677584031,2.4375009535537249,1.8370701339216138,1.0476572157368238,0,1.7953601756358306,0,1.9194020852823617,2.5560504567642366,1.6855600412224936,0,2.7492910464848208,5.3319755221002945,1.5503391582355153,1.8015979630722911]
["BV650-A",2.0994162415286075,1.0393399754809129,2.2122476298437332,0,0.53072908275388664,1.1288753119354467,0.54853931845863202,0.54021149716695105,0,0.29801363944107911,0,0,0.33064055800975656,2.5198132802886657,2.865085196055023,2.0980740310327022,1.072110382027434,0,0.44903832423253631,0.99098179001374442,0,3.1622508518556107,2.2720087219686658,0,0,0.91704860449550807,1.0188303589322283,1.0855940991113329]
["BV711-A",0.57852345735791311,2.010665714692752,3.5621742599258379,0,0.25499789657883004,0.40958312980713346,1.0976908150618112,1.7443516776922807,0.50224771845161253,0.34185831633541913,0,0,0,0.60174283574493215,3.8237617105856372,3.2669221364511851,0.60835361710721358,0,0,0,0.67379503695017517,0,2.5670813299233295,0.57829665176652079,0,0,0.15422649199159683,1.1810625461440878]
["BV750-A",0,1.9395463066525462,1.8547642377710982,0,0.24441668271358669,0.20813695023841272,0.59219550223370609,2.4990666327977036,1.0182991938610932,0.43076439741891104,0,8.195089419781006E-3,0,0,3.6832516854588615,4.3995993011785037,0.44359330063591473,0,0,0,0.24285153613778365,0.98085362469046555,0,1.0656334700000458,0,0,0,1.1023019120198629]
["BV786-A",0.45778612933834828,3.086474240306452,1.0728768363527634,0,0.52964088725401315,1.9659388579332381E-2,0.50026042102812396,2.2562007729880516,1.4897791043875481,0.96409362448354685,0,0,0.40173628948909224,2.7763668354477695E-2,2.6602544619377717,10.116680842632041,1.0301272425307837,0,0,0,1.1906816056437961,0.71734826516603389,4.7742547988457043,0,0,0.58586411747730294,0,2.1191974406822949]
["PE-A",0.57332350034767476,0.12408733900206327,0.1627257942649695,0,2.098375280838761,1.2875728518569749,0.99442921026186615,0.9597196955980396,0.34024672641720233,0.17964395140107986,0,0.63560410779684051,0.34442400245177895,0.30800170699646179,0.31717492544192666,0.25058138216611642,0.31204721657451934,0,0.9112735967089266,0.67543674821684885,0.46086651328496675,0.48263600568302545,1.8227553274365378E-2,0,0,3.4408734499117801,0.84093291259611613,0.83518725469912103]
["PE-CF594-A",0.9334912398171108,0.16326753528486096,0.44104915954064172,0,3.3100106593299485,2.406554208219621,1.7879548905422102,1.8759723881885828,0.75099910406712966,1.5215715236388929E-3,0,9.5134666737957618E-2,0.38152133472623107,0.49033082631699965,0.50211234684035178,0.42433520472127118,0.16314805404439223,0,0.44670935952817437,0.86393820064194127,0.63545807598969195,0.63223033228061354,0.46263769735990895,0,1.5813048985442346,0,1.5628977116761547,1.6260753604272782]
["PE-Cy5-A",3.45497534866798,1.2632283435714726,2.2984758799487799,0,0.30157737861946687,4.5487457137763885,3.811166180107997,3.5285599375315782,1.4557687838041053,0.15251460042000486,0,0,0,1.2260985996214202,0.95796103346568573,0.77768652226371271,0.27068357908593121,0,0,0.24316381799683706,1.1772464680958536,1.2440196682694478,0.77291971473857857,0,0.45521054034523811,0.41200764815957186,0,3.3650091568632234]
["PE-Cy7-A",0,0.76279876292039361,0.20431356933031253,0,0.1965623029074377,0.13687088501470518,0.46997304907740844,2.7587400393831203,2.4747180943406244,0.12840373653079465,0,0,0,9.2475810801735919E-2,0.36388309395626317,1.4692960802651045,0.12454671643347305,0,0,0,0,0.10836349246745182,0.73525543072043342,0.18564146461248815,0.25023083150564834,0.2182704266998533,0,0]
[]
["Spread Matrix based on 1:100 diluted CD4 single stains"]

{"sheet":"CD4 1_500","index":2,"n_rows":32,"n_cols":29,"rows_kept":32,"cut":false,"hidden":true,"merged":[]}
["Column1","Column2","Column3","Column4","Column24","Column5","Column6","Column29","Column7","Column8","Column9","Column10","Column11","Column12","Column13","Column14","Column15","Column16","Column17","Column18","Column19","Column20","Column21","Column22","Column23","Column25","Column26","Column27","Column28"]
["SSM","APC-A","APC-Cy7-A","APC-R700-A","BB515-A","BB630-A","BB660-A","BB700-A","BB755-A","BB790-A","BUV395-A","BUV496-A","BUV563-A","BUV615-A","BUV661-A","BUV737-A","BUV805-A","BV421-A","BV480-A","BV570-A","BV605-A","BV650-A","BV711-A","BV750-A","BV786-A","PE-A","PE-CF594-A","PE-Cy5-A","PE-Cy7-A"]
["APC-A",0,2.0231677455739372,3.2677598384343614,0,0,1.7211560289342966,0.57129130931451366,0,0.47616493045341912,0,0,0,0,0.91514855893060498,1.1249618352471078,7.9548323418358394E-2,0,0,0,0,0.60797467691118479,0.4175377354102266,0.58045627451517978,0.79382183071362833,0,3.3739009601915879E-2,1.7929057982136152,1.6439332355123439]
["APC-Cy7-A",0.84291498234551265,0,0.8589584171142326,0,0,2.1148969821246109E-2,0.35921984151260361,0,0.70730705869096888,0,0,0,0,0,0.56499118204147569,1.5499258744088054,0,8.7675921139785153E-3,0,0,0.38134109914153613,0,1.1475676055196373,0.78253797798960856,0,0,0,2.9915361894664172]
["APC-R700-A",0.71571547282567383,2.5211621037488432,0,0,0,0,0.53040875016495215,0,0.31985272390848868,0,0,0,0,0,1.1670567148874944,1.6373460911355708,0,0,0,0,0,0.4890149668228439,0.89368609530922305,0.41171398556989242,0,1.3406843009753941E-2,0.18386637419462537,1.9304308123498157]
["BB515-A",0,0,0,0,0.78724364536910207,0.33891047315145328,0,0,0,0.31321028967623638,0,0,0,0,0,0.53580461987252637,0,0,0,0,0,0,0.99822434833933149,0.66029910450280849,0,0.77693383735271326,0.29461954033732779,0]
["BB630-A",1.2629932325312581,0,0.53432263793419765,0,0,2.341322820391984,1.9450992706893686,1.4257757259671828,0.71464829114903128,0,0,0,0.55730726203187186,0.80666644307783686,0.73308740008550344,0.93805392211522198,0.26820870336733638,0.10342532684803341,0.61117376238348076,1.4518816555763658,1.1264874079487659,0.85135694834194908,0.85293590934536823,0.58289300684224521,0.98790599849897265,2.8753373943786698,0.96552636530129643,0.78499365499789653]
["BB660-A",2.813152810952384,1.4117223047026797,2.7888496648182755,0,0,0,3.4125155719790934,2.5397246606642825,1.2493303560719424,0,0,0,0,1.0888451401470003,1.2523572729473671,0.75954260308220722,0.25198137735283815,0,0,0,1.5476611741616926,1.4840569631505494,1.0049662695125972,0.78778355662857835,0,0,1.0670244442109635,0.94356463174910943]
["BB700-A",1.4197058286741235,1.7266449981531951,2.821558760792406,0,0.25602728159500016,2.4830332664207955,0,3.7214892109549678,1.9579034681565306,0,0,0,0,0.68408972142885183,1.5462621340467706,1.7792391256367979,0.24613034985949325,0,0,0,1.0033697453781927,2.0789815217399634,1.9810509761295778,0.21349995581419368,0,0,0.55285157043404098,1.1881118886367539]
["BB755-A",0,1.6863242072512614,0.9817608680655654,0,0.86694445115064234,0.9826498341215405,1.8430253063929654,0,3.2718581239097144,0,0,0,0,0,1.784274123499312,2.04809617237026,0,6.9030656630528148E-3,0,0,0.52966282607430892,0.6686228423392131,2.3210492581582627,0.61854864071785198,0,0,9.1129196252414373E-3,1.2639254972010303]
["BB790-A",0.27783976978403857,1.1744546428892331,0,0,0.31039044877491967,0.3839297118555941,1.2232444117097159,4.4201757572157003,0,0.3557424556392878,0,0,0,1.2382394743167623E-2,1.0657540418037135,3.9841646829084643,0,0,0,0,0,0,1.9701155071437786,1.0698339001397097,0,0,0,1.2443207689677809]
["BUV395-A",0.44046501020377887,0.49610943399536606,0,0,0,0,0,0,0,0,0,0,0,0,0,0.25046912613142625,0,0,0,0,0,0,0,1.7042738767272989,0,3.9083988744945918E-2,0,0.90559407974184003]
["BUV496-A",0,1.4933678596840296,0,0,1.8267714150650147,0,1.0051233512685012,0,0,2.8103127474414293,0,1.229839857560078,0,0,0,0,0,0,0,0,2.1743859328222488,0,2.9559487342219812,0,0,0.16569610907402285,0,1.3507332275836117]
["BUV563-A",0.92967442869132544,0.73502921570297841,0,0,2.1422188537241302,0,0.99932638333793966,0,0.96468491895766495,1.1986317782582629,0,0,1.007885411576767,1.0922547903148014,1.4358529116466765,2.6550025225572349,0,0,0,0,5.5695392143245051E-2,0,0.84412312442549575,0,3.1389065076019786,3.4936685167286052,1.4010391210035704,1.3424052709231746]
["BUV615-A",1.6430573446725649,5.404537402841765E-2,0.87554242053977582,0,3.523767538040671,1.7178347027739571,0,0,1.2695274349458467,1.1052167204180192,0,0,0,2.563089072553915,3.4770121877920324,2.4481839767285716,0,1.5740335289304595E-2,0,0,0.98543593899546211,0,0.77587278712765928,0.80389779953022711,2.3367811698985546,5.8999725468526352,2.238518533442694,2.3244184991546915]
["BUV661-A",2.9250332024887542,1.7201554508349781,3.0851272882526746,0,0.54294964619318231,1.0340364022246664,0,0,0,0.77325680091699522,0,0,0,0,3.6714787375994775,2.7508583935391004,0,0,0,0,0.97616862646605373,0,0.43409219357464018,0.79651923018815052,0,0,1.120245808658793,1.577145721431392]
["BUV737-A",0,1.9367641315696338,2.7788708258717767,0,0,0.30405367089552227,0.81432430419416391,1.4202500674258562,0.86478819506781224,0.49784632976553517,0,0,0,0,0,3.6230292837636853,0,0.21453863949762866,0,0,0,0,0.94918978123049302,0.41508914550238291,0,0,0,1.1407435059923521]
["BUV805-A",0,0.95437536393555811,0,0,0.503451765564502,0,0,0,0,0.89043337211482243,0,0,0,0,0.69175755423231211,0,0,0,0,0,2.6826516702611727E-2,0,3.0267036295260192E-2,1.0470778605978408,0,0,0,0.45440545643389535]
["BV421-A",0,0,0,0,0,0,0,0,0,0,0,0,0,4.0723269015439205E-2,0.427710548820883,0,0,0.5388413309682536,0,0,1.157813635075889,0,0,1.2027972537682967,0,0,0,0]
["BV480-A",0,0,0,0,0,0.44689561040753678,0,0,0,0,0,0.42444584844816097,0,0,0.62674641756025806,0,0.9127514998860502,0,0.99334830544121888,0.71143086462060245,0,0,8.9445081056675202E-2,1.7650331268539894,0,0,0,0.80963298011367291]
["BV570-A",0,0,0,0,1.6947286514146449,1.706581369865882,0,0,0,1.1057707556193581,0,0,0,1.1707720749132557,0,0,1.0661655691440024,1.0443326018348793,0,0,1.7271389233325587,0.96955988524550796,2.4786341251719897,0,3.3451401399249407,2.4992636744187284,0.73170431450199724,1.9896794742061246]
["BV605-A",0,0,0,0,1.4603438750886304,2.0433207622151763,0,0,0,0,0,0,0,2.0046855320950252,2.7694442577822485,1.4274975309154938,0,0,0,0,3.2597737756011815,9.618243479990847E-2,1.6544411518197946,3.3157029930199045,2.1373898506370024,4.0219105400842352,1.7884759881679231,2.7220218313525679]
["BV650-A",1.8883033626512247,1.0796626711409842,1.9218527799306213,0,0,1.1613172309057689,0.89068102310672359,0,0.49213831070162301,0,0,0,0,2.0866760931297814,2.9785216593279129,1.7901347255675863,1.1342512712841886,0,0,0.51709394729433056,0,3.2713039762576273,2.2478262062001169,0.90335010543028693,0,1.6386193353547094,1.2707361908606494,1.4058386799575944]
["BV711-A",0.68212506906745762,1.7100265944648989,3.0366132488870732,0,0,0.39715582873542021,1.0278331691604945,0.91499419062838427,0.60694304874845517,0.29880179164597742,0,0,0,0.60738033606183806,3.6876576213433943,3.2554722309450983,0.41387402866722434,0,0,0,1.0886092698017731,0,2.9812737627072186,1.0625815683047535,0,0.42550193911728312,0,1.0455651897462599]
["BV750-A",0,1.7922026597121887,1.736720343029869,0,0,0,0,1.7786308655094145,0.8299427335460009,0.26465360598697502,0,0,0,0,3.3604884950625968,4.6663004861338768,0.36455011408222965,7.6730909781987516E-3,0,0,0.47895333188313116,0.88557844948435938,0,0.79215505606760017,0,0,0,1.1336321486648817]
["BV786-A",0,2.7372469306143161,0.8070162017831507,0,1.2602976279911826,0,0.84684139538464454,0,1.4340315837256514,0,0,0,0,0,2.6526483862267702,8.8196187494236629,0.97787989820715682,0.54133994910533645,0,0,0,0,4.8586739019729395,0,0,1.0138941125094381,0,1.4022756797098259]
["PE-A",0.6429192307316367,0.30993410013007106,0.28180578489923802,0,1.8277031281192686,1.3007410882856265,1.0097292323242013,0.38871391611578004,0.35353877146610196,0,0,0.45260981994630434,0.17162988206152641,0.42371868786053984,0.47113925942032503,1.0853268145651533,0,0,0.60850640448433724,0.61872902716404254,0.64836882995698453,0.21871055288732175,0.66526666916781085,0,0,3.1296324202481811,0.94780217914391685,0.87246052989709721]
["PE-CF594-A",0.98042740943910012,0,0.42024859150292848,0,2.7881469373474586,2.1729542419840882,1.7114469698269787,1.1745313002150395,0.70953454616854894,0,0,0,0.28435721545733617,0.55834342797270509,0.52009354792191864,0.68802205130425653,0,0,0.34529198656212251,0.62988040064824835,0.60983615958053317,0.66365570377879224,0.74785004672048538,0,1.556772317698724,0,1.5378133593537044,1.6017431380615386]
["PE-Cy5-A",2.4324357074954066,1.2271471567752301,2.2172456009607178,0,0.31652620376583801,3.9991857206156602,3.3512940289614113,2.6113590821056296,1.3828836423332529,0,0,0,0,0.96394790706503419,1.0199130023384608,0.97338703434940432,0.1204412251201751,0,1.9734572777140049E-2,0,1.0189430679321219,1.0668365140813199,1.0040632573663346,0.32506817324895287,0.38527863185746253,0.40227695977129446,0,3.2456915000979634]
["PE-Cy7-A",0.20381723422984588,0.66286057743025573,0,0,0,0,0,1.7476808592760396,2.1675139386772737,0,0,0,0,0,0.27852446428888278,1.392626241657771,0,0,0,0,0.23129388663120207,3.0446269971402564E-2,1.0043276860880985,0.38615158399407579,0,0.45411497604772222,0.2455178897878087,0]
[]
["Spread Matrix based on 1:500 diluted CD4 single stains"]

{"sheet":"CD4 Spread Table","index":3,"n_rows":58,"n_cols":34,"rows_kept":58,"cut":false,"hidden":false,"merged":[[57,2,57,31],[58,2,58,31],[3,3,4,23],[47,1,55,1],[47,2,50,31],[52,2,54,31],[55,2,55,31]]}
[]
[null,null,null,"Total Spread Matrix Visualization Tool"]
[]
[null,null,null,"Designed to support the optimization of higher parameter flow cytometry panel design. \n\nDeveloped at the European Centre for Single Cell Solutions (ESS) in Heidelberg by Jens Fleischer, Annette Meyer, and Mark Dessing \nwith Microsoft Excel optimization by Kwinten de Temmerman & Amelia Uznanska"]
[]
[]
[]
[null,"Scroll down for instructions or email:",null,null,"help.biosciences@bd.com",null,null,null,null,"Free text fields [user defined]",null,null,null,null,null,"Adjustable fields",null,null,null,null,null,"Total Spread < 300",null,null,null,null,null,"Total Spread >300"]
[]
[null,null,null,"Your Panel",{"$f":"B12","v":"CD marker tbd"},{"$f":"B13","v":"CD marker tbd"},{"$f":"B15","v":"CD marker tbd"},{"$f":"B16","v":"CD marker tbd"},{"$f":"B18","v":"CD marker tbd"},{"$f":"B19","v":"CD marker tbd"},{"$f":"B21","v":"CD marker tbd"},{"$f":"B22","v":"CD marker tbd"},{"$f":"B23","v":"CD marker tbd"},{"$f":"B24","v":"CD marker tbd"},{"$f":"B25","v":"CD marker tbd"},{"$f":"B26","v":"CD marker tbd"},{"$f":"B27","v":"CD marker tbd"},{"$f":"B28","v":"CD marker tbd"},{"$f":"B29","v":"CD marker tbd"},{"$f":"B30","v":"CD marker tbd"},{"$f":"B31","v":"CD marker tbd"},{"$f":"B32","v":"CD marker tbd"},{"$f":"B34","v":"CD marker tbd"},{"$f":"B35","v":"CD marker tbd"},{"$f":"B36","v":"CD marker tbd"},{"$f":"B37","v":"CD marker tbd"},{"$f":"B38","v":"CD marker tbd"},{"$f":"B39","v":"CD marker tbd"},{"$f":"B40","v":"CD marker tbd"},{"$f":"B41","v":"CD marker tbd"},{"$f":"B42","v":"CD marker tbd"},{"$f":"B43","v":"CD marker tbd"}]
[null,"Your Panel","Density (Select)","Fluorescent Dye","APC","APC-R700","APC-Cy7","BB515","BB630","BB660","BB700","BB755","BB790","BUV395","BUV496","BUV563","BUV615","BUV661","BUV737","BUV805","BV421","BV480","BV570","BV605","BV650","BV711","BV750","BV786","PE","PE-CF594","PE-Cy5","PE-Cy7","Visualize a dye spread","Stain Index"]
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[null,"CD marker tbd","High","Alexa700",{"$f":"IF(E$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!B4,$C14=\"Med\",MED!B4,$C14=\"Low\",LOW!B4))","v":33.011800000000001},{"$f":"IF(F$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!C4,$C14=\"Med\",MED!C4,$C14=\"Low\",LOW!C4))","v":0},{"$f":"IF(G$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!D4,$C14=\"Med\",MED!D4,$C14=\"Low\",LOW!D4))","v":279.95800000000003},{"$f":"IF(H$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!E4,$C14=\"Med\",MED!E4,$C14=\"Low\",LOW!E4))","v":0},{"$f":"IF(I$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!F4,$C14=\"Med\",MED!F4,$C14=\"Low\",LOW!F4))","v":2.2286000000000001},{"$f":"IF(J$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!G4,$C14=\"Med\",MED!G4,$C14=\"Low\",LOW!G4))","v":17.628499999999999},{"$f":"IF(K$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!H4,$C14=\"Med\",MED!H4,$C14=\"Low\",LOW!H4))","v":55.5732},{"$f":"IF(L$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!I4,$C14=\"Med\",MED!I4,$C14=\"Low\",LOW!I4))","v":96.237799999999993},{"$f":"IF(M$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!J4,$C14=\"Med\",MED!J4,$C14=\"Low\",LOW!J4))","v":39.868200000000002},{"$f":"IF(N$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!K4,$C14=\"Med\",MED!K4,$C14=\"Low\",LOW!K4))","v":12.1091},{"$f":"IF(O$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!L4,$C14=\"Med\",MED!L4,$C14=\"Low\",LOW!L4))","v":0},{"$f":"IF(P$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!M4,$C14=\"Med\",MED!M4,$C14=\"Low\",LOW!M4))","v":11.946099999999999},{"$f":"IF(Q$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!N4,$C14=\"Med\",MED!N4,$C14=\"Low\",LOW!N4))","v":20.029699999999998},{"$f":"IF(R$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!O4,$C14=\"Med\",MED!O4,$C14=\"Low\",LOW!O4))","v":28.293500000000002},{"$f":"IF(S$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!P4,$C14=\"Med\",MED!P4,$C14=\"Low\",LOW!P4))","v":146.315},{"$f":"IF(T$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!Q4,$C14=\"Med\",MED!Q4,$C14=\"Low\",LOW!Q4))","v":160.87200000000001},{"$f":"IF(U$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!R4,$C14=\"Med\",MED!R4,$C14=\"Low\",LOW!R4))","v":20.572099999999999},{"$f":"IF(V$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!S4,$C14=\"Med\",MED!S4,$C14=\"Low\",LOW!S4))","v":0},{"$f":"IF(W$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!T4,$C14=\"Med\",MED!T4,$C14=\"Low\",LOW!T4))","v":0},{"$f":"IF(X$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!U4,$C14=\"Med\",MED!U4,$C14=\"Low\",LOW!U4))","v":0},{"$f":"IF(Y$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!V4,$C14=\"Med\",MED!V4,$C14=\"Low\",LOW!V4))","v":26.4558},{"$f":"IF(Z$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!W4,$C14=\"Med\",MED!W4,$C14=\"Low\",LOW!W4))","v":77.351299999999995},{"$f":"IF(AA$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!X4,$C14=\"Med\",MED!X4,$C14=\"Low\",LOW!X4))","v":91.475899999999996},{"$f":"IF(AB$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!Y4,$C14=\"Med\",MED!Y4,$C14=\"Low\",LOW!Y4))","v":35.390500000000003},{"$f":"IF(AC$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!Z4,$C14=\"Med\",MED!Z4,$C14=\"Low\",LOW!Z4))","v":18.867999999999999},{"$f":"IF(AD$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!AA4,$C14=\"Med\",MED!AA4,$C14=\"Low\",LOW!AA4))","v":34.088500000000003},{"$f":"IF(AE$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!AB4,$C14=\"Med\",MED!AB4,$C14=\"Low\",LOW!AB4))","v":23.5487},{"$f":"IF(AF$11=$D14,\"-\",_xlfn.IFS($C14=\"High\",HIGH!AC4,$C14=\"Med\",MED!AC4,$C14=\"Low\",LOW!AC4))","v":191.297},"Alexa700 Spread",103]
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[null,"CD marker tbd","Low","PE",{"$f":"IF(E$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!B30,$C40=\"Med\",MED!B30,$C40=\"Low\",LOW!B30))","v":45.709000000000003},{"$f":"IF(F$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!C30,$C40=\"Med\",MED!C30,$C40=\"Low\",LOW!C30))","v":13.476800000000001},{"$f":"IF(G$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!D30,$C40=\"Med\",MED!D30,$C40=\"Low\",LOW!D30))","v":20.736999999999998},{"$f":"IF(H$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!E30,$C40=\"Med\",MED!E30,$C40=\"Low\",LOW!E30))","v":0},{"$f":"IF(I$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!F30,$C40=\"Med\",MED!F30,$C40=\"Low\",LOW!F30))","v":124.146},{"$f":"IF(J$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!G30,$C40=\"Med\",MED!G30,$C40=\"Low\",LOW!G30))","v":86.825699999999998},{"$f":"IF(K$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!H30,$C40=\"Med\",MED!H30,$C40=\"Low\",LOW!H30))","v":68.177999999999997},{"$f":"IF(L$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!I30,$C40=\"Med\",MED!I30,$C40=\"Low\",LOW!I30))","v":21.362500000000001},{"$f":"IF(M$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!J30,$C40=\"Med\",MED!J30,$C40=\"Low\",LOW!J30))","v":23.865100000000002},{"$f":"IF(N$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!K30,$C40=\"Med\",MED!K30,$C40=\"Low\",LOW!K30))","v":0},{"$f":"IF(O$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!L30,$C40=\"Med\",MED!L30,$C40=\"Low\",LOW!L30))","v":0},{"$f":"IF(P$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!M30,$C40=\"Med\",MED!M30,$C40=\"Low\",LOW!M30))","v":30.543399999999998},{"$f":"IF(Q$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!N30,$C40=\"Med\",MED!N30,$C40=\"Low\",LOW!N30))","v":11.715299999999999},{"$f":"IF(R$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!O30,$C40=\"Med\",MED!O30,$C40=\"Low\",LOW!O30))","v":25.4419},{"$f":"IF(S$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!P30,$C40=\"Med\",MED!P30,$C40=\"Low\",LOW!P30))","v":31.813400000000001},{"$f":"IF(T$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!Q30,$C40=\"Med\",MED!Q30,$C40=\"Low\",LOW!Q30))","v":66.153800000000004},{"$f":"IF(U$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!R30,$C40=\"Med\",MED!R30,$C40=\"Low\",LOW!R30))","v":0},{"$f":"IF(V$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!S30,$C40=\"Med\",MED!S30,$C40=\"Low\",LOW!S30))","v":0.343225},{"$f":"IF(W$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!T30,$C40=\"Med\",MED!T30,$C40=\"Low\",LOW!T30))","v":43.323700000000002},{"$f":"IF(X$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!U30,$C40=\"Med\",MED!U30,$C40=\"Low\",LOW!U30))","v":38.902799999999999},{"$f":"IF(Y$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!V30,$C40=\"Med\",MED!V30,$C40=\"Low\",LOW!V30))","v":40.838799999999999},{"$f":"IF(Z$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!W30,$C40=\"Med\",MED!W30,$C40=\"Low\",LOW!W30))","v":14.7166},{"$f":"IF(AA$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!X30,$C40=\"Med\",MED!X30,$C40=\"Low\",LOW!X30))","v":44.055999999999997},{"$f":"IF(AB$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!Y30,$C40=\"Med\",MED!Y30,$C40=\"Low\",LOW!Y30))","v":17.467500000000001},{"$f":"IF(AC$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!Z30,$C40=\"Med\",MED!Z30,$C40=\"Low\",LOW!Z30))","v":"-"},{"$f":"IF(AD$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!AA30,$C40=\"Med\",MED!AA30,$C40=\"Low\",LOW!AA30))","v":212.453},{"$f":"IF(AE$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!AB30,$C40=\"Med\",MED!AB30,$C40=\"Low\",LOW!AB30))","v":59.6982},{"$f":"IF(AF$11=$D40,\"-\",_xlfn.IFS($C40=\"High\",HIGH!AC30,$C40=\"Med\",MED!AC30,$C40=\"Low\",LOW!AC30))","v":53.655999999999999},"PE Spread",380]
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[null,"CD marker tbd","Low","PE-Cy5",{"$f":"IF(E$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!B32,$C42=\"Med\",MED!B32,$C42=\"Low\",LOW!B32))","v":232.83799999999999},{"$f":"IF(F$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!C32,$C42=\"Med\",MED!C32,$C42=\"Low\",LOW!C32))","v":206.548},{"$f":"IF(G$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!D32,$C42=\"Med\",MED!D32,$C42=\"Low\",LOW!D32))","v":118.488},{"$f":"IF(H$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!E32,$C42=\"Med\",MED!E32,$C42=\"Low\",LOW!E32))","v":0},{"$f":"IF(I$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!F32,$C42=\"Med\",MED!F32,$C42=\"Low\",LOW!F32))","v":21.1629},{"$f":"IF(J$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!G32,$C42=\"Med\",MED!G32,$C42=\"Low\",LOW!G32))","v":377.79},{"$f":"IF(K$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!H32,$C42=\"Med\",MED!H32,$C42=\"Low\",LOW!H32))","v":321.096},{"$f":"IF(L$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!I32,$C42=\"Med\",MED!I32,$C42=\"Low\",LOW!I32))","v":246.77099999999999},{"$f":"IF(M$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!J32,$C42=\"Med\",MED!J32,$C42=\"Low\",LOW!J32))","v":130.75899999999999},{"$f":"IF(N$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!K32,$C42=\"Med\",MED!K32,$C42=\"Low\",LOW!K32))","v":0},{"$f":"IF(O$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!L32,$C42=\"Med\",MED!L32,$C42=\"Low\",LOW!L32))","v":0},{"$f":"IF(P$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!M32,$C42=\"Med\",MED!M32,$C42=\"Low\",LOW!M32))","v":0},{"$f":"IF(Q$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!N32,$C42=\"Med\",MED!N32,$C42=\"Low\",LOW!N32))","v":0},{"$f":"IF(R$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!O32,$C42=\"Med\",MED!O32,$C42=\"Low\",LOW!O32))","v":89.569199999999995},{"$f":"IF(S$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!P32,$C42=\"Med\",MED!P32,$C42=\"Low\",LOW!P32))","v":93.384600000000006},{"$f":"IF(T$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!Q32,$C42=\"Med\",MED!Q32,$C42=\"Low\",LOW!Q32))","v":88.312200000000004},{"$f":"IF(U$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!R32,$C42=\"Med\",MED!R32,$C42=\"Low\",LOW!R32))","v":16.040500000000002},{"$f":"IF(V$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!S32,$C42=\"Med\",MED!S32,$C42=\"Low\",LOW!S32))","v":0.343225},{"$f":"IF(W$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!T32,$C42=\"Med\",MED!T32,$C42=\"Low\",LOW!T32))","v":1.47224},{"$f":"IF(X$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!U32,$C42=\"Med\",MED!U32,$C42=\"Low\",LOW!U32))","v":0},{"$f":"IF(Y$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!V32,$C42=\"Med\",MED!V32,$C42=\"Low\",LOW!V32))","v":98.0535},{"$f":"IF(Z$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!W32,$C42=\"Med\",MED!W32,$C42=\"Low\",LOW!W32))","v":102.474},{"$f":"IF(AA$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!X32,$C42=\"Med\",MED!X32,$C42=\"Low\",LOW!X32))","v":91.518900000000002},{"$f":"IF(AB$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!Y32,$C42=\"Med\",MED!Y32,$C42=\"Low\",LOW!Y32))","v":43.283099999999997},{"$f":"IF(AC$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!Z32,$C42=\"Med\",MED!Z32,$C42=\"Low\",LOW!Z32))","v":36.107199999999999},{"$f":"IF(AD$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!AA32,$C42=\"Med\",MED!AA32,$C42=\"Low\",LOW!AA32))","v":38.012300000000003},{"$f":"IF(AE$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!AB32,$C42=\"Med\",MED!AB32,$C42=\"Low\",LOW!AB32))","v":"-"},{"$f":"IF(AF$11=$D42,\"-\",_xlfn.IFS($C42=\"High\",HIGH!AC32,$C42=\"Med\",MED!AC32,$C42=\"Low\",LOW!AC32))","v":303.49599999999998},"PE-Cy5 Spread",718]
[null,"CD marker tbd","Low","PE-Cy7",{"$f":"IF(E$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!B33,$C43=\"Med\",MED!B33,$C43=\"Low\",LOW!B33))","v":13.162800000000001},{"$f":"IF(F$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!C33,$C43=\"Med\",MED!C33,$C43=\"Low\",LOW!C33))","v":0},{"$f":"IF(G$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!D33,$C43=\"Med\",MED!D33,$C43=\"Low\",LOW!D33))","v":47.778199999999998},{"$f":"IF(H$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!E33,$C43=\"Med\",MED!E33,$C43=\"Low\",LOW!E33))","v":0},{"$f":"IF(I$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!F33,$C43=\"Med\",MED!F33,$C43=\"Low\",LOW!F33))","v":0},{"$f":"IF(J$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!G33,$C43=\"Med\",MED!G33,$C43=\"Low\",LOW!G33))","v":0},{"$f":"IF(K$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!H33,$C43=\"Med\",MED!H33,$C43=\"Low\",LOW!H33))","v":0},{"$f":"IF(L$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!I33,$C43=\"Med\",MED!I33,$C43=\"Low\",LOW!I33))","v":111.05200000000001},{"$f":"IF(M$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!J33,$C43=\"Med\",MED!J33,$C43=\"Low\",LOW!J33))","v":139.82300000000001},{"$f":"IF(N$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!K33,$C43=\"Med\",MED!K33,$C43=\"Low\",LOW!K33))","v":0},{"$f":"IF(O$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!L33,$C43=\"Med\",MED!L33,$C43=\"Low\",LOW!L33))","v":0},{"$f":"IF(P$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!M33,$C43=\"Med\",MED!M33,$C43=\"Low\",LOW!M33))","v":0},{"$f":"IF(Q$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!N33,$C43=\"Med\",MED!N33,$C43=\"Low\",LOW!N33))","v":0},{"$f":"IF(R$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!O33,$C43=\"Med\",MED!O33,$C43=\"Low\",LOW!O33))","v":0},{"$f":"IF(S$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!P33,$C43=\"Med\",MED!P33,$C43=\"Low\",LOW!P33))","v":17.975300000000001},{"$f":"IF(T$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!Q33,$C43=\"Med\",MED!Q33,$C43=\"Low\",LOW!Q33))","v":88.6447},{"$f":"IF(U$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!R33,$C43=\"Med\",MED!R33,$C43=\"Low\",LOW!R33))","v":0},{"$f":"IF(V$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!S33,$C43=\"Med\",MED!S33,$C43=\"Low\",LOW!S33))","v":0},{"$f":"IF(W$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!T33,$C43=\"Med\",MED!T33,$C43=\"Low\",LOW!T33))","v":0},{"$f":"IF(X$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!U33,$C43=\"Med\",MED!U33,$C43=\"Low\",LOW!U33))","v":0},{"$f":"IF(Y$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!V33,$C43=\"Med\",MED!V33,$C43=\"Low\",LOW!V33))","v":14.9595},{"$f":"IF(Z$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!W33,$C43=\"Med\",MED!W33,$C43=\"Low\",LOW!W33))","v":2.15774},{"$f":"IF(AA$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!X33,$C43=\"Med\",MED!X33,$C43=\"Low\",LOW!X33))","v":63.6571},{"$f":"IF(AB$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!Y33,$C43=\"Med\",MED!Y33,$C43=\"Low\",LOW!Y33))","v":39.316800000000001},{"$f":"IF(AC$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!Z33,$C43=\"Med\",MED!Z33,$C43=\"Low\",LOW!Z33))","v":0},{"$f":"IF(AD$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!AA33,$C43=\"Med\",MED!AA33,$C43=\"Low\",LOW!AA33))","v":29.276900000000001},{"$f":"IF(AE$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!AB33,$C43=\"Med\",MED!AB33,$C43=\"Low\",LOW!AB33))","v":11.266400000000001},{"$f":"IF(AF$11=$D43,\"-\",_xlfn.IFS($C43=\"High\",HIGH!AC33,$C43=\"Med\",MED!AC33,$C43=\"Low\",LOW!AC33))","v":"-"},"PE-Cy7 Spread",428]
[]
[]
[]
[]
[null,"Instructions \nfor use","Clicking the hyperlink in column AG brings up a page of plots featuring population stains at three levels of antigen density with the representative spread into other channels.\nTo achieve this, each respective dye conjugated anti-CD4 mAb was used at the recommended concentration as well as a 1:100 and 1:500 dilution to achieve a high, medium and low stain respectively.\nEach page of plots features a \"Back to table\" button, which will bring you back here.\n\nUse the information presented to determine whether any given spread potentially impacts stain resolution in critical channels allocated for dim antigen resolution. \nAdjust your expected antigen density and assess whether the staining intensity affects other dim markers."]
[]
[]
[]
[]
[null,null,"Hovering the cursor above any cell in the matrix with a red triangle (in the top right corner) brings up a plot illustrating spread for that specific dye combination.\nFields without this red triangle do not exhibit any visible signs of spread. The respective plots can however be viewed via the hyperlinks in column AG.\nYou may need to scroll or adjust your zoom to see the full plot.\n"]
[]
[]
[null,null,"Additional details please see \"Instrument and Setup Details\" Tab."]
[]
[null,"Note","Data generated in association to this tool is simply intended to guide optimization of a panel under development. \nBD does not warrant that the performance of a panel designed with this tool would not require further modification following the first test with biological samples. \nIt is the responsibility of the user to decide whether the data generated meets their expectation or if further optimization efforts are required.\n\nFor Research Use Only. Not for use in diagnostic or therapeutic procedures. "]

{"sheet":"HIGH","index":4,"n_rows":33,"n_cols":29,"rows_kept":33,"cut":false,"hidden":true,"merged":[]}
[null,"APC","APC-R700","APC-Cy7","BB515","BB630","BB660","BB700","BB755","BB790","BUV395","BUV496","BUV563","BUV615","BUV661","BUV737","BUV805","BV421-A","BV480-A","BV570","BV605","BV650","BV711","BV750","BV786-A","PE","PE-CF594","PE-Cy5","PE-Cy7"]
["APC",0,442.88900000000001,251.77,0,27.134699999999999,197.529,56.670299999999997,60.22,27.709,0,0,0,11.4017,133.87200000000001,130.749,90.975300000000004,23.3078,0,0,0,85.310100000000006,88.157200000000003,76.206699999999998,0,0,29.2684,234.86799999999999,209.41]
["APC-R700-A",168.22900000000001,0,558.42899999999997,0,0,89.995099999999994,131.20699999999999,171.125,89.074700000000007,24.8931,0,0.74374099999999999,0,55.845399999999998,321.44499999999999,207.61099999999999,20.083400000000001,0,0,19.7211,43.520400000000002,137.691,128.90100000000001,43.487000000000002,0,23.8279,79.490300000000005,445.85700000000003]
["Alexa700",33.011800000000001,0,279.95800000000003,0,2.2286000000000001,17.628499999999999,55.5732,96.237799999999993,39.868200000000002,12.1091,0,11.946099999999999,20.029699999999998,28.293500000000002,146.315,160.87200000000001,20.572099999999999,0,0,0,26.4558,77.351299999999995,91.475899999999996,35.390500000000003,18.867999999999999,34.088500000000003,23.5487,191.297]
["APC-Cy7",83.799400000000006,183.73699999999999,0,0,15.6561,51.3416,29.183299999999999,110.021,124.95399999999999,35.946599999999997,0,0,16.246200000000002,28.579000000000001,81.971100000000007,371.82600000000002,20.1068,0,0,0,40.487099999999998,47.511499999999998,97.775499999999994,0,0,16.795000000000002,45.121099999999998,636.92999999999995]
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["BV570",41.3583,34.168599999999998,25.702000000000002,0,127.02800000000001,86.839799999999997,54.3795,75.483900000000006,37.080399999999997,0,0,110.211,51.667099999999998,88.062700000000007,73.604299999999995,83.362399999999994,111.825,0,0,144.12700000000001,125.36199999999999,121.18300000000001,34.917400000000001,0,235.80600000000001,241.35599999999999,76.444000000000003,85.638099999999994]
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["PE-CF594",261.79599999999999,114.30200000000001,48.139800000000001,0,1029.73,596.92499999999995,488.738,494.32799999999997,192.53899999999999,12.248799999999999,0,24.7242,120.047,131.185,155.00399999999999,117.768,48.0989,0,107.935,269.83,166.648,161.53800000000001,116.544,39.587699999999998,412.88900000000001,0,421.28800000000001,436.91500000000002]
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{"sheet":"MED","index":5,"n_rows":33,"n_cols":29,"rows_kept":33,"cut":false,"hidden":true,"merged":[]}
["Total Spread Matrix","APC-A","APCR700","APC-Cy7-A","BB515","BB630-A","BB660-A","BB700","BB755-A","BB790-A","BUV395-A","BUV496-A","BUV563-A","BUV615-A","BUV661-A","BUV737-A","BUV805-A","BV421-A","BV510-A","BV570-A","BV605-A","BV650-A","BV711-A","BV750-A","BV786-A","PE-A","PE-CF594-A","PE-Cy5-A","PE-Cy7-A"]
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["APCR700",75.2851,0,272.05799999999999,0,0,44.932299999999998,50.068600000000004,87.531199999999998,47.6663,0,0,0,0,28.599499999999999,141.18600000000001,86.187799999999996,11.564299999999999,0,0,0,0,66.925600000000003,47.226999999999997,0,0,0,39.301600000000001,218.547]
["Alexa700-A",35.790399999999998,0,284.798,0,0,12.4428,50.314999999999998,102.089,54.4696,0,0,0,0,12.3689,151.34700000000001,106.366,0,0,0,0,37.6417,75.491100000000003,83.412400000000005,30.424700000000001,0,23.6997,20.0441,177.553]
["APC-Cy7-A",62.632599999999996,80.4893,0,0,0,35.166200000000003,0,38.726300000000002,54.4514,0.244723,0,0,0,12.586600000000001,48.823,180.08600000000001,0,0,0,0,21.659099999999999,2.0255200000000002,47.259099999999997,0,0,16.842500000000001,22.692699999999999,287.46699999999998]
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["FITC-A",18.6844,0,25.6614,0,43.657600000000002,41.0623,36.495800000000003,58.167400000000001,27.935500000000001,0,0,0,0,17.6327,29.1723,0,11.8314,0,0,0,37.603499999999997,21.328299999999999,0,0,0,0.99953000000000003,0,1.4263399999999999]
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["PerCP-Cy5-5-A",92.917000000000002,166.613,97.978899999999996,0,15.3842,134.41800000000001,0,270.44400000000002,113.64,0,0,0,11.563499999999999,71.193600000000004,132.77799999999999,126.742,37.289400000000001,0,13.867100000000001,0,113.39100000000001,189.709,127.685,0,0,0,88.456100000000006,172.441]
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["BV570-A",18.8736,0,33.165199999999999,0,38.163499999999999,35.166200000000003,0,0,0,9.9903900000000004E-2,0,24.6294,20.277699999999999,36.730400000000003,37.449199999999998,0,36.391500000000001,0,0,38.908900000000003,47.189599999999999,30.052800000000001,0,0,99.721999999999994,105.242,32.921799999999998,38.915199999999999]
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["BUV563",0.7491485465438521,0.33334295599660158,0,0,1.6533479790141696,0.85734205649592998,0.46477741577497289,0.9443204090798164,0,1.0084988872191241,0,0,1.0578590399395846,1.338478264181939,1.3321841541319137,1.0605754724244558,0,0,0,0,0.69129980859909645,3.0466259336318292E-2,0,0,3.7381553951638291,3.8170207734252211,0.85441329377222897,0.9947713141129727]
["BUV615",2.0373695030623979,0.55824498988733218,0.97563438652276824,0,3.6389541598576782,2.1446918534114929,0.91871320811758206,1.2668052005898915,0.75630812628639077,0.88099759666677768,0,0.53963900610399573,0,2.9129510113035249,3.0350055037488497,1.992264897880633,0,0,0,0.96708281653323269,0.96034307655670015,1.1477269017468088,0.76657557534813359,0,2.2010609110534038,7.1733891327987571,2.114030732191778,2.3636284338900548]
["BUV661",4.2626002767553599,1.9205054699728556,3.0719235687240039,0,3.7438956570493856E-2,1.2477761031252061,0.33162489028661951,0.72894530985480555,0.18179726698052173,0.83176368474155016,0,0.22748612838412549,0.26685560767487188,0,3.609513360449137,2.5793773410497329,0.15490840059933175,0,0,0,0.77751488647459654,0.65818389000242405,0.78028133127322163,0,0,0.67910782720566132,1.2315928503627434,1.3810682730684771]
["BUV737",0.24280249708033397,2.1634525371931805,4.0772549335968797,0,0,0.23846513210860576,1.0382485925489524,2.4005500552317667,0.89077601511861226,0.57445580733451285,0,0,0.1050890329502312,0.22914957107701314,0,4.0284203842135904,0,0,0,0,0.13641804566051288,0.38306770651806582,0.87864602610673082,0,0,0.15280211061224006,0,1.0229284408347918]
["BUV805",0,1.1770752076062501,0.36692293575461382,0,0.33299721604958393,0,0.2239890166068054,0.59560579037967243,0.43334672392534357,0.89841321836729338,0,0,0.17888065868082303,0.33701472798002047,0.28219135890407931,0,0,0,0,0,0,0,0,0,0,2.4451077294940674E-2,0,0.8830243455040413]
["BV421",0,0.30176319475338886,0,0,0.31345861121011287,0.21737112161502561,0,0.26042631773778147,0,0,0,0,0.19460859824477422,0.20871133689660146,0.30731536534898213,0,0,0,0,0,0.31065059876156514,0.34730245637739271,0,0,0,0,0,0.22398461488080776]
["BV480",0,2.6001708164212815E-2,0,0.18652305033957406,0,1.500009512580563E-2,0,0,0.25653074263745773,0.56728224147891571,0.22631211123259287,0.51716474609681407,0.43819320580179705,0.67666490216350117,0.6485546463946803,0,0.44550755942929043,0,1.2966916308414138,0.77244578127395824,0.91730578068755675,0.67367092139759521,0.99271236302651344,0,0.6776396870666993,0.70747117140829252,0,0.56474431619891552]
["BV570",1.0909770594704686,1.0757666717466214,0,0,1.600073732841008,1.2796901667253957,0,0.56128254809868616,0,7.5153780272472492E-3,0,0.89750245710073084,0.73762015039193674,1.4945046146073226,1.2683098826551866,0,1.2563016016904007,0,0,1.296204541235167,1.8865415887529535,1.1996461798923952,1.2725108479058866,0,3.5389715137213638,3.6949380464305901,1.1272467474255272,1.318445661527744]
["BV605",1.2881953819207859,1.2951669389008171,0.52904365533457653,0,2.5820782950600516,1.7516140661533377,0.80054618301895453,1.0497572814043696,0,8.0566312416658105E-3,0,0.82937784203185883,1.0324213460903298,1.8534921677584031,2.4375009535537249,1.8370701339216138,1.0476572157368238,0,1.7953601756358306,0,1.9194020852823617,2.5560504567642366,1.6855600412224936,0,2.7492910464848208,5.3319755221002945,1.5503391582355153,1.8015979630722911]
["BV650",2.0994162415286075,1.0393399754809129,2.2122476298437332,0,0.53072908275388664,1.1288753119354467,0.54853931845863202,0.54021149716695105,0,0.29801363944107911,0,0,0.33064055800975656,2.5198132802886657,2.865085196055023,2.0980740310327022,1.072110382027434,0,0.44903832423253631,0.99098179001374442,0,3.1622508518556107,2.2720087219686658,0,0,0.91704860449550807,1.0188303589322283,1.0855940991113329]
["BV711",0.57852345735791311,2.010665714692752,3.5621742599258379,0,0.25499789657883004,0.40958312980713346,1.0976908150618112,1.7443516776922807,0.50224771845161253,0.34185831633541913,0,0,0,0.60174283574493215,3.8237617105856372,3.2669221364511851,0.60835361710721358,0,0,0,0.67379503695017517,0,2.5670813299233295,0.57829665176652079,0,0,0.15422649199159683,1.1810625461440878]
["BV750",0,1.9395463066525462,1.8547642377710982,0,0.24441668271358669,0.20813695023841272,0.59219550223370609,2.4990666327977036,1.0182991938610932,0.43076439741891104,0,8.195089419781006E-3,0,0,3.6832516854588615,4.3995993011785037,0.44359330063591473,0,0,0,0.24285153613778365,0.98085362469046555,0,1.0656334700000458,0,0,0,1.1023019120198629]
["BV786",0.45778612933834828,3.086474240306452,1.0728768363527634,0,0.52964088725401315,1.9659388579332381E-2,0.50026042102812396,2.2562007729880516,1.4897791043875481,0.96409362448354685,0,0,0.40173628948909224,2.7763668354477695E-2,2.6602544619377717,10.116680842632041,1.0301272425307837,0,0,0,1.1906816056437961,0.71734826516603389,4.7742547988457043,0,0,0.58586411747730294,0,2.1191974406822949]
["PE",0.57332350034767476,0.12408733900206327,0.1627257942649695,0,2.098375280838761,1.2875728518569749,0.99442921026186615,0.9597196955980396,0.34024672641720233,0.17964395140107986,0,0.63560410779684051,0.34442400245177895,0.30800170699646179,0.31717492544192666,0.25058138216611642,0.31204721657451934,0,0.9112735967089266,0.67543674821684885,0.46086651328496675,0.48263600568302545,1.8227553274365378E-2,0,0,3.4408734499117801,0.84093291259611613,0.83518725469912103]
["PE-CF594",0.9334912398171108,0.16326753528486096,0.44104915954064172,0,3.3100106593299485,2.406554208219621,1.7879548905422102,1.8759723881885828,0.75099910406712966,1.5215715236388929E-3,0,9.5134666737957618E-2,0.38152133472623107,0.49033082631699965,0.50211234684035178,0.42433520472127118,0.16314805404439223,0,0.44670935952817437,0.86393820064194127,0.63545807598969195,0.63223033228061354,0.46263769735990895,0,1.5813048985442346,0,1.5628977116761547,1.6260753604272782]
["PE-Cy5",3.45497534866798,1.2632283435714726,2.2984758799487799,0,0.30157737861946687,4.5487457137763885,3.811166180107997,3.5285599375315782,1.4557687838041053,0.15251460042000486,0,0,0,1.2260985996214202,0.95796103346568573,0.77768652226371271,0.27068357908593121,0,0,0.24316381799683706,1.1772464680958536,1.2440196682694478,0.77291971473857857,0,0.45521054034523811,0.41200764815957186,0,3.3650091568632234]
["PE-Cy7",0,0.76279876292039361,0.20431356933031253,0,0.1965623029074377,0.13687088501470518,0.46997304907740844,2.7587400393831203,2.4747180943406244,0.12840373653079465,0,0,0,9.2475810801735919E-2,0.36388309395626317,1.4692960802651045,0.12454671643347305,0,0,0,0,0.10836349246745182,0.73525543072043342,0.18564146461248815,0.25023083150564834,0.2182704266998533,0,0]
[]
["Spread Matrix based on 1:100 diluted CD4 single stains"]
[]
[]
[null,null,null,null,null,null,"Spread above 6"]
[]
[null,null,null,null,null,null,"Spread between 3 and 6"]
[]
[null,null,null,null,null,null,"Spread below 3"]

{"sheet":"CD4 1_500 square","index":8,"n_rows":38,"n_cols":29,"rows_kept":38,"cut":false,"hidden":true,"merged":[]}
["Column1","Column2","Column3","Column4","Column24","Column5","Column6","Column29","Column7","Column8","Column9","Column10","Column11","Column12","Column13","Column14","Column15","Column16","Column17","Column18","Column19","Column20","Column21","Column22","Column23","Column25","Column26","Column27","Column28"]
["SSM","APC","APC-Cy7","APC-R700","BB515","BB630","BB660","BB700","BB755","BB790","BUV395","BUV496","BUV563","BUV615","BUV661","BUV737","BUV805","BV421","BV480","BV570","BV605","BV650","BV711","BV750","BV786","PE","PE-CF594","PE-Cy5","PE-Cy7"]
["APC",0,2.0231677455739372,3.2677598384343614,0,0,1.7211560289342966,0.57129130931451366,0,0.47616493045341912,0,0,0,0,0.91514855893060498,1.1249618352471078,7.9548323418358394E-2,0,0,0,0,0.60797467691118479,0.4175377354102266,0.58045627451517978,0.79382183071362833,0,3.3739009601915879E-2,1.7929057982136152,1.6439332355123439]
["APC-Cy7",0.84291498234551265,0,0.8589584171142326,0,0,2.1148969821246109E-2,0.35921984151260361,0,0.70730705869096888,0,0,0,0,0,0.56499118204147569,1.5499258744088054,0,8.7675921139785153E-3,0,0,0.38134109914153613,0,1.1475676055196373,0.78253797798960856,0,0,0,2.9915361894664172]
["APC-R700",0.71571547282567383,2.5211621037488432,0,0,0,0,0.53040875016495215,0,0.31985272390848868,0,0,0,0,0,1.1670567148874944,1.6373460911355708,0,0,0,0,0,0.4890149668228439,0.89368609530922305,0.41171398556989242,0,1.3406843009753941E-2,0.18386637419462537,1.9304308123498157]
["BB515",0,0,0,0,0.78724364536910207,0.33891047315145328,0,0,0,0.31321028967623638,0,0,0,0,0,0.53580461987252637,0,0,0,0,0,0,0.99822434833933149,0.66029910450280849,0,0.77693383735271326,0.29461954033732779,0]
["BB630",1.2629932325312581,0,0.53432263793419765,0,0,2.341322820391984,1.9450992706893686,1.4257757259671828,0.71464829114903128,0,0,0,0.55730726203187186,0.80666644307783686,0.73308740008550344,0.93805392211522198,0.26820870336733638,0.10342532684803341,0.61117376238348076,1.4518816555763658,1.1264874079487659,0.85135694834194908,0.85293590934536823,0.58289300684224521,0.98790599849897265,2.8753373943786698,0.96552636530129643,0.78499365499789653]
["BB660",2.813152810952384,1.4117223047026797,2.7888496648182755,0,0,0,3.4125155719790934,2.5397246606642825,1.2493303560719424,0,0,0,0,1.0888451401470003,1.2523572729473671,0.75954260308220722,0.25198137735283815,0,0,0,1.5476611741616926,1.4840569631505494,1.0049662695125972,0.78778355662857835,0,0,1.0670244442109635,0.94356463174910943]
["BB700",1.4197058286741235,1.7266449981531951,2.821558760792406,0,0.25602728159500016,2.4830332664207955,0,3.7214892109549678,1.9579034681565306,0,0,0,0,0.68408972142885183,1.5462621340467706,1.7792391256367979,0.24613034985949325,0,0,0,1.0033697453781927,2.0789815217399634,1.9810509761295778,0.21349995581419368,0,0,0.55285157043404098,1.1881118886367539]
["BB755",0,1.6863242072512614,0.9817608680655654,0,0.86694445115064234,0.9826498341215405,1.8430253063929654,0,3.2718581239097144,0,0,0,0,0,1.784274123499312,2.04809617237026,0,6.9030656630528148E-3,0,0,0.52966282607430892,0.6686228423392131,2.3210492581582627,0.61854864071785198,0,0,9.1129196252414373E-3,1.2639254972010303]
["BB790",0.27783976978403857,1.1744546428892331,0,0,0.31039044877491967,0.3839297118555941,1.2232444117097159,4.4201757572157003,0,0.3557424556392878,0,0,0,1.2382394743167623E-2,1.0657540418037135,3.9841646829084643,0,0,0,0,0,0,1.9701155071437786,1.0698339001397097,0,0,0,1.2443207689677809]
["BUV395",0.44046501020377887,0.49610943399536606,0,0,0,0,0,0,0,0,0,0,0,0,0,0.25046912613142625,0,0,0,0,0,0,0,1.7042738767272989,0,3.9083988744945918E-2,0,0.90559407974184003]
["BUV496",0,1.4933678596840296,0,0,1.8267714150650147,0,1.0051233512685012,0,0,2.8103127474414293,0,1.229839857560078,0,0,0,0,0,0,0,0,2.1743859328222488,0,2.9559487342219812,0,0,0.16569610907402285,0,1.3507332275836117]
["BUV563",0.92967442869132544,0.73502921570297841,0,0,2.1422188537241302,0,0.99932638333793966,0,0.96468491895766495,1.1986317782582629,0,0,1.007885411576767,1.0922547903148014,1.4358529116466765,2.6550025225572349,0,0,0,0,5.5695392143245051E-2,0,0.84412312442549575,0,3.1389065076019786,3.4936685167286052,1.4010391210035704,1.3424052709231746]
["BUV615",1.6430573446725649,5.404537402841765E-2,0.87554242053977582,0,3.523767538040671,1.7178347027739571,0,0,1.2695274349458467,1.1052167204180192,0,0,0,2.563089072553915,3.4770121877920324,2.4481839767285716,0,1.5740335289304595E-2,0,0,0.98543593899546211,0,0.77587278712765928,0.80389779953022711,2.3367811698985546,5.8999725468526352,2.238518533442694,2.3244184991546915]
["BUV661",2.9250332024887542,1.7201554508349781,3.0851272882526746,0,0.54294964619318231,1.0340364022246664,0,0,0,0.77325680091699522,0,0,0,0,3.6714787375994775,2.7508583935391004,0,0,0,0,0.97616862646605373,0,0.43409219357464018,0.79651923018815052,0,0,1.120245808658793,1.577145721431392]
["BUV737",0,1.9367641315696338,2.7788708258717767,0,0,0.30405367089552227,0.81432430419416391,1.4202500674258562,0.86478819506781224,0.49784632976553517,0,0,0,0,0,3.6230292837636853,0,0.21453863949762866,0,0,0,0,0.94918978123049302,0.41508914550238291,0,0,0,1.1407435059923521]
["BUV805",0,0.95437536393555811,0,0,0.503451765564502,0,0,0,0,0.89043337211482243,0,0,0,0,0.69175755423231211,0,0,0,0,0,2.6826516702611727E-2,0,3.0267036295260192E-2,1.0470778605978408,0,0,0,0.45440545643389535]
["BV421",0,0,0,0,0,0,0,0,0,0,0,0,0,4.0723269015439205E-2,0.427710548820883,0,0,0.5388413309682536,0,0,1.157813635075889,0,0,1.2027972537682967,0,0,0,0]
["BV480",0,0,0,0,0,0.44689561040753678,0,0,0,0,0,0.42444584844816097,0,0,0.62674641756025806,0,0.9127514998860502,0,0.99334830544121888,0.71143086462060245,0,0,8.9445081056675202E-2,1.7650331268539894,0,0,0,0.80963298011367291]
["BV570",0,0,0,0,1.6947286514146449,1.706581369865882,0,0,0,1.1057707556193581,0,0,0,1.1707720749132557,0,0,1.0661655691440024,1.0443326018348793,0,0,1.7271389233325587,0.96955988524550796,2.4786341251719897,0,3.3451401399249407,2.4992636744187284,0.73170431450199724,1.9896794742061246]
["BV605",0,0,0,0,1.4603438750886304,2.0433207622151763,0,0,0,0,0,0,0,2.0046855320950252,2.7694442577822485,1.4274975309154938,0,0,0,0,3.2597737756011815,9.618243479990847E-2,1.6544411518197946,3.3157029930199045,2.1373898506370024,4.0219105400842352,1.7884759881679231,2.7220218313525679]
["BV650",1.8883033626512247,1.0796626711409842,1.9218527799306213,0,0,1.1613172309057689,0.89068102310672359,0,0.49213831070162301,0,0,0,0,2.0866760931297814,2.9785216593279129,1.7901347255675863,1.1342512712841886,0,0,0.51709394729433056,0,3.2713039762576273,2.2478262062001169,0.90335010543028693,0,1.6386193353547094,1.2707361908606494,1.4058386799575944]
["BV711",0.68212506906745762,1.7100265944648989,3.0366132488870732,0,0,0.39715582873542021,1.0278331691604945,0.91499419062838427,0.60694304874845517,0.29880179164597742,0,0,0,0.60738033606183806,3.6876576213433943,3.2554722309450983,0.41387402866722434,0,0,0,1.0886092698017731,0,2.9812737627072186,1.0625815683047535,0,0.42550193911728312,0,1.0455651897462599]
["BV750",0,1.7922026597121887,1.736720343029869,0,0,0,0,1.7786308655094145,0.8299427335460009,0.26465360598697502,0,0,0,0,3.3604884950625968,4.6663004861338768,0.36455011408222965,7.6730909781987516E-3,0,0,0.47895333188313116,0.88557844948435938,0,0.79215505606760017,0,0,0,1.1336321486648817]
["BV786",0,2.7372469306143161,0.8070162017831507,0,1.2602976279911826,0,0.84684139538464454,0,1.4340315837256514,0,0,0,0,0,2.6526483862267702,8.8196187494236629,0.97787989820715682,0.54133994910533645,0,0,0,0,4.8586739019729395,0,0,1.0138941125094381,0,1.4022756797098259]
["PE",0.6429192307316367,0.30993410013007106,0.28180578489923802,0,1.8277031281192686,1.3007410882856265,1.0097292323242013,0.38871391611578004,0.35353877146610196,0,0,0.45260981994630434,0.17162988206152641,0.42371868786053984,0.47113925942032503,1.0853268145651533,0,0,0.60850640448433724,0.61872902716404254,0.64836882995698453,0.21871055288732175,0.66526666916781085,0,0,3.1296324202481811,0.94780217914391685,0.87246052989709721]
["PE-CF594",0.98042740943910012,0,0.42024859150292848,0,2.7881469373474586,2.1729542419840882,1.7114469698269787,1.1745313002150395,0.70953454616854894,0,0,0,0.28435721545733617,0.55834342797270509,0.52009354792191864,0.68802205130425653,0,0,0.34529198656212251,0.62988040064824835,0.60983615958053317,0.66365570377879224,0.74785004672048538,0,1.556772317698724,0,1.5378133593537044,1.6017431380615386]
["PE-Cy5",2.4324357074954066,1.2271471567752301,2.2172456009607178,0,0.31652620376583801,3.9991857206156602,3.3512940289614113,2.6113590821056296,1.3828836423332529,0,0,0,0,0.96394790706503419,1.0199130023384608,0.97338703434940432,0.1204412251201751,0,1.9734572777140049E-2,0,1.0189430679321219,1.0668365140813199,1.0040632573663346,0.32506817324895287,0.38527863185746253,0.40227695977129446,0,3.2456915000979634]
["PE-Cy7",0.20381723422984588,0.66286057743025573,0,0,0,0,0,1.7476808592760396,2.1675139386772737,0,0,0,0,0,0.27852446428888278,1.392626241657771,0,0,0,0,0.23129388663120207,3.0446269971402564E-2,1.0043276860880985,0.38615158399407579,0,0.45411497604772222,0.2455178897878087,0]
[]
["Spread Matrix based on 1:500 diluted CD4 single stains"]
[]
[null,null,null,null,null,null,"Spread above 6"]
[]
[null,null,null,null,null,null,"Spread between 3 and 6"]
[]
[null,null,null,null,null,null,"Spread below 3"]

{"sheet":"CD4 1_500 JF BD presentations","index":9,"n_rows":32,"n_cols":29,"rows_kept":32,"cut":false,"hidden":true,"merged":[]}
["Column1","Column2","Column3","Column4","Column24","Column5","Column6","Column29","Column7","Column8","Column9","Column10","Column11","Column12","Column13","Column14","Column15","Column16","Column17","Column18","Column19","Column20","Column21","Column22","Column23","Column25","Column26","Column27","Column28"]
["SSM","APC-A","APC-Cy7-A","APC-R700-A","BB515-A","BB630-A","BB660-A","BB700-A","BB755-A","BB790-A","BUV395-A","BUV496-A","BUV563-A","BUV615-A","BUV661-A","BUV737-A","BUV805-A","BV421-A","BV480-A","BV570-A","BV605-A","BV650-A","BV711-A","BV750-A","BV786-A","PE-A","PE-CF594-A","PE-Cy5-A","PE-Cy7-A"]
["APC-A",0,2.0231677455739372,3.2677598384343614,0,0,1.7211560289342966,0.57129130931451366,0,0.47616493045341912,0,0,0,0,0.91514855893060498,1.1249618352471078,7.9548323418358394E-2,0,0,0,0,0.60797467691118479,0.4175377354102266,0.58045627451517978,0.79382183071362833,0,3.3739009601915879E-2,1.7929057982136152,1.6439332355123439]
["APC-Cy7-A",0.84291498234551265,0,0.8589584171142326,0,0,2.1148969821246109E-2,0.35921984151260361,0,0.70730705869096888,0,0,0,0,0,0.56499118204147569,1.5499258744088054,0,8.7675921139785153E-3,0,0,0.38134109914153613,0,1.1475676055196373,0.78253797798960856,0,0,0,2.9915361894664172]
["APC-R700-A",0.71571547282567383,2.5211621037488432,0,0,0,0,0.53040875016495215,0,0.31985272390848868,0,0,0,0,0,1.1670567148874944,1.6373460911355708,0,0,0,0,0,0.4890149668228439,0.89368609530922305,0.41171398556989242,0,1.3406843009753941E-2,0.18386637419462537,1.9304308123498157]
["BB515-A",0,0,0,0,0.78724364536910207,0.33891047315145328,0,0,0,0.31321028967623638,0,0,0,0,0,0.53580461987252637,0,0,0,0,0,0,0.99822434833933149,0.66029910450280849,0,0.77693383735271326,0.29461954033732779,0]
["BB630-A",1.2629932325312581,0,0.53432263793419765,0,0,2.341322820391984,1.9450992706893686,1.4257757259671828,0.71464829114903128,0,0,0,0.55730726203187186,0.80666644307783686,0.73308740008550344,0.93805392211522198,0.26820870336733638,0.10342532684803341,0.61117376238348076,1.4518816555763658,1.1264874079487659,0.85135694834194908,0.85293590934536823,0.58289300684224521,0.98790599849897265,2.8753373943786698,0.96552636530129643,0.78499365499789653]
["BB660-A",2.813152810952384,1.4117223047026797,2.7888496648182755,0,0,0,3.4125155719790934,2.5397246606642825,1.2493303560719424,0,0,0,0,1.0888451401470003,1.2523572729473671,0.75954260308220722,0.25198137735283815,0,0,0,1.5476611741616926,1.4840569631505494,1.0049662695125972,0.78778355662857835,0,0,1.0670244442109635,0.94356463174910943]
["BB700-A",1.4197058286741235,1.7266449981531951,2.821558760792406,0,0.25602728159500016,2.4830332664207955,0,3.7214892109549678,1.9579034681565306,0,0,0,0,0.68408972142885183,1.5462621340467706,1.7792391256367979,0.24613034985949325,0,0,0,1.0033697453781927,2.0789815217399634,1.9810509761295778,0.21349995581419368,0,0,0.55285157043404098,1.1881118886367539]
["BB755-A",0,1.6863242072512614,0.9817608680655654,0,0.86694445115064234,0.9826498341215405,1.8430253063929654,0,3.2718581239097144,0,0,0,0,0,1.784274123499312,2.04809617237026,0,6.9030656630528148E-3,0,0,0.52966282607430892,0.6686228423392131,2.3210492581582627,0.61854864071785198,0,0,9.1129196252414373E-3,1.2639254972010303]
["BB790-A",0.27783976978403857,1.1744546428892331,0,0,0.31039044877491967,0.3839297118555941,1.2232444117097159,4.4201757572157003,0,0.3557424556392878,0,0,0,1.2382394743167623E-2,1.0657540418037135,3.9841646829084643,0,0,0,0,0,0,1.9701155071437786,1.0698339001397097,0,0,0,1.2443207689677809]
["BUV395-A",0.44046501020377887,0.49610943399536606,0,0,0,0,0,0,0,0,0,0,0,0,0,0.25046912613142625,0,0,0,0,0,0,0,1.7042738767272989,0,3.9083988744945918E-2,0,0.90559407974184003]
["BUV496-A",0,1.4933678596840296,0,0,1.8267714150650147,0,1.0051233512685012,0,0,2.8103127474414293,0,1.229839857560078,0,0,0,0,0,0,0,0,2.1743859328222488,0,2.9559487342219812,0,0,0.16569610907402285,0,1.3507332275836117]
["BUV563-A",0.92967442869132544,0.73502921570297841,0,0,2.1422188537241302,0,0.99932638333793966,0,0.96468491895766495,1.1986317782582629,0,0,1.007885411576767,1.0922547903148014,1.4358529116466765,2.6550025225572349,0,0,0,0,5.5695392143245051E-2,0,0.84412312442549575,0,3.1389065076019786,3.4936685167286052,1.4010391210035704,1.3424052709231746]
["BUV615-A",1.6430573446725649,5.404537402841765E-2,0.87554242053977582,0,3.523767538040671,1.7178347027739571,0,0,1.2695274349458467,1.1052167204180192,0,0,0,2.563089072553915,3.4770121877920324,2.4481839767285716,0,1.5740335289304595E-2,0,0,0.98543593899546211,0,0.77587278712765928,0.80389779953022711,2.3367811698985546,5.8999725468526352,2.238518533442694,2.3244184991546915]
["BUV661-A",2.9250332024887542,1.7201554508349781,3.0851272882526746,0,0.54294964619318231,1.0340364022246664,0,0,0,0.77325680091699522,0,0,0,0,3.6714787375994775,2.7508583935391004,0,0,0,0,0.97616862646605373,0,0.43409219357464018,0.79651923018815052,0,0,1.120245808658793,1.577145721431392]
["BUV737-A",0,1.9367641315696338,2.7788708258717767,0,0,0.30405367089552227,0.81432430419416391,1.4202500674258562,0.86478819506781224,0.49784632976553517,0,0,0,0,0,3.6230292837636853,0,0.21453863949762866,0,0,0,0,0.94918978123049302,0.41508914550238291,0,0,0,1.1407435059923521]
["BUV805-A",0,0.95437536393555811,0,0,0.503451765564502,0,0,0,0,0.89043337211482243,0,0,0,0,0.69175755423231211,0,0,0,0,0,2.6826516702611727E-2,0,3.0267036295260192E-2,1.0470778605978408,0,0,0,0.45440545643389535]
["BV421-A",0,0,0,0,0,0,0,0,0,0,0,0,0,4.0723269015439205E-2,0.427710548820883,0,0,0.5388413309682536,0,0,1.157813635075889,0,0,1.2027972537682967,0,0,0,0]
["BV480-A",0,0,0,0,0,0.44689561040753678,0,0,0,0,0,0.42444584844816097,0,0,0.62674641756025806,0,0.9127514998860502,0,0.99334830544121888,0.71143086462060245,0,0,8.9445081056675202E-2,1.7650331268539894,0,0,0,0.80963298011367291]
["BV570-A",0,0,0,0,1.6947286514146449,1.706581369865882,0,0,0,1.1057707556193581,0,0,0,1.1707720749132557,0,0,1.0661655691440024,1.0443326018348793,0,0,1.7271389233325587,0.96955988524550796,2.4786341251719897,0,3.3451401399249407,2.4992636744187284,0.73170431450199724,1.9896794742061246]
["BV605-A",0,0,0,0,1.4603438750886304,2.0433207622151763,0,0,0,0,0,0,0,2.0046855320950252,2.7694442577822485,1.4274975309154938,0,0,0,0,3.2597737756011815,9.618243479990847E-2,1.6544411518197946,3.3157029930199045,2.1373898506370024,4.0219105400842352,1.7884759881679231,2.7220218313525679]
["BV650-A",1.8883033626512247,1.0796626711409842,1.9218527799306213,0,0,1.1613172309057689,0.89068102310672359,0,0.49213831070162301,0,0,0,0,2.0866760931297814,2.9785216593279129,1.7901347255675863,1.1342512712841886,0,0,0.51709394729433056,0,3.2713039762576273,2.2478262062001169,0.90335010543028693,0,1.6386193353547094,1.2707361908606494,1.4058386799575944]
["BV711-A",0.68212506906745762,1.7100265944648989,3.0366132488870732,0,0,0.39715582873542021,1.0278331691604945,0.91499419062838427,0.60694304874845517,0.29880179164597742,0,0,0,0.60738033606183806,3.6876576213433943,3.2554722309450983,0.41387402866722434,0,0,0,1.0886092698017731,0,2.9812737627072186,1.0625815683047535,0,0.42550193911728312,0,1.0455651897462599]
["BV750-A",0,1.7922026597121887,1.736720343029869,0,0,0,0,1.7786308655094145,0.8299427335460009,0.26465360598697502,0,0,0,0,3.3604884950625968,4.6663004861338768,0.36455011408222965,7.6730909781987516E-3,0,0,0.47895333188313116,0.88557844948435938,0,0.79215505606760017,0,0,0,1.1336321486648817]
["BV786-A",0,2.7372469306143161,0.8070162017831507,0,1.2602976279911826,0,0.84684139538464454,0,1.4340315837256514,0,0,0,0,0,2.6526483862267702,8.8196187494236629,0.97787989820715682,0.54133994910533645,0,0,0,0,4.8586739019729395,0,0,1.0138941125094381,0,1.4022756797098259]
["PE-A",0.6429192307316367,0.30993410013007106,0.28180578489923802,0,1.8277031281192686,1.3007410882856265,1.0097292323242013,0.38871391611578004,0.35353877146610196,0,0,0.45260981994630434,0.17162988206152641,0.42371868786053984,0.47113925942032503,1.0853268145651533,0,0,0.60850640448433724,0.61872902716404254,0.64836882995698453,0.21871055288732175,0.66526666916781085,0,0,3.1296324202481811,0.94780217914391685,0.87246052989709721]
["PE-CF594-A",0.98042740943910012,0,0.42024859150292848,0,2.7881469373474586,2.1729542419840882,1.7114469698269787,1.1745313002150395,0.70953454616854894,0,0,0,0.28435721545733617,0.55834342797270509,0.52009354792191864,0.68802205130425653,0,0,0.34529198656212251,0.62988040064824835,0.60983615958053317,0.66365570377879224,0.74785004672048538,0,1.556772317698724,0,1.5378133593537044,1.6017431380615386]
["PE-Cy5-A",2.4324357074954066,1.2271471567752301,2.2172456009607178,0,0.31652620376583801,3.9991857206156602,3.3512940289614113,2.6113590821056296,1.3828836423332529,0,0,0,0,0.96394790706503419,1.0199130023384608,0.97338703434940432,0.1204412251201751,0,1.9734572777140049E-2,0,1.0189430679321219,1.0668365140813199,1.0040632573663346,0.32506817324895287,0.38527863185746253,0.40227695977129446,0,3.2456915000979634]
["PE-Cy7-A",0.20381723422984588,0.66286057743025573,0,0,0,0,0,1.7476808592760396,2.1675139386772737,0,0,0,0,0,0.27852446428888278,1.392626241657771,0,0,0,0,0.23129388663120207,3.0446269971402564E-2,1.0043276860880985,0.38615158399407579,0,0.45411497604772222,0.2455178897878087,0]
[]
["Spread Matrix based on 1:500 diluted CD4 single stains"]

{"sheet":"Instrument and Setup Details","index":10,"n_rows":55,"n_cols":7,"rows_kept":55,"cut":false,"hidden":false,"merged":[[46,1,46,6],[50,1,50,6],[53,1,53,6],[51,1,51,6]]}
[]
[null,"LASER","POWER","DETECTOR","PARAMETERS","BANDPASS","DICHROIC"]
[]
[null,488,"200mW","6 Colors (PMTs)"]
[null,null,null,"A","BB790","810/40","770LP"]
[null,null,null,"B","BB755","750/30","735LP"]
[null,null,null,"C","BB700, PerCP-Cy5.5","710/50","685LP"]
[null,null,null,"D","BB660","670/30","635LP"]
[null,null,null,"E","BB630","610/20","600LP"]
[null,null,null,"F  ","BB515, FITC","530/30","505LP"]
[null,null,null,"G","SSC","488/10","n.a."]
[]
[null,405,"150mW","8 Colors (PMTs)"]
[null,null,null,"A","BV786","810/40","770LP"]
[null,null,null,"B","BV750","750/30","735LP"]
[null,null,null,"C","BV711","710/50","685LP"]
[null,null,null,"D","BV650","677/20","635LP"]
[null,null,null,"E","BV605","605/40","595LP"]
[null,null,null,"F","BV570","586/15","550LP"]
[null,null,null,"G","BV510, BV480","525/50","505LP"]
[null,null,null,"H  ","BV421","431/28","410LP"]
[]
[null,355,"60mW","7 Colors (PMTs)"]
[null,null,null,"A","BUV805","810/40","770LP"]
[null,null,null,"B","BUV737","735/30","690LP"]
[null,null,null,"C","BUV661","670/25","630LP"]
[null,null,null,"D","BUV615","610/20","600LP"]
[null,null,null,"E","BUV563","580/20","550LP"]
[null,null,null,"F","BUV496","515/30","490LP"]
[null,null,null,"G","DAPI/UV440","450/50","410LP"]
[null,null,null,"H","BUV395","379/28","n.a."]
[]
[null,637,"140mW","3 Colors (PMTs)"]
[null,null,null,"A","APC-Cy7","780/60","750LP"]
[null,null,null,"B","APC-R700, Alexa700","730/45","690LP"]
[null,null,null,"C","APC","670/30","655LP"]
[]
[null,561,"150mW","4 Colors (PMTs)"]
[null,null,null,"A","PE-Cy7","780/60","750LP"]
[null,null,null,"B","PE-Cy5","670/30","635LP"]
[null,null,null,"C","PE-CF594","610/20","600LP"]
[null,null,null,"D  ","PE","586/15","570LP"]
[]
[]
[null,"PMT setup procedure"]
[]
[null,"Antibodies used: \nCD4 clone SK3 (also known as Leu3a) from the BD Human Fluorchrochrome evaluation kit, Catalogue number 566352.\n1.5µl of each mAb (Undiluted, diluted 1:100 and diluted 1:500) was used to stain 1 million PBMC obtained from a healthy volunteer donor. \n1:100 and 1:500 dilutions of the stock mAbs were prepared using BD Stain buffer catalogue number 554656.\n\nAll negative populations from the unstained tube were set to a mean value of 100.\nEach single tube was then run to check for dynamic range and intensity.\n\nWhere the signal in the primary channel was found to be less than that in neighbouring channels, adjustments were made to the voltage (balancing), to obtain the maximal signal in the respective channels.\n\nFollowing this initial check, unstained and single color control samples were recorded in BD FACSDivaTM software and compensation was then calculated.\n\nDatasets were imported into FlowJoTM software to generate the total spread matrices (TSM) for the three different mAb dilutions. \n\nThe compensation matrix and the total spread matrix were calculated for each data set independently.\n\n"]
[]
[null,"The Stain index shown was calculated as published"]
[]
[null,"Maciorowski et.al. Basic Multicolor Flow Cytometry. Current Protocols in Immunology 2017   "]
[null,"https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpim.26"]
[]
[null,"TSM data presented in this tool are derived from a single experiment.\n\nNote: TSM data collected on other BD FACSymphonyTM A5 (of similar specification; 5 Laser, 28 fluorescent parameter) and BD FACSymphonyTM A3 instruments (standard European specification: 5 Laser, 28 fluorescent parameter) have generated comparable results. Data not presented here."]
[null,"For Research Use Only. Not for use in diagnostic or therapeutic procedures. "]

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