These are some of the techniques we use in the lab:

  • Quantification of chromatin interactions: 3C-seq, 4C-seq, Hi-C.
  • Genome editing: CRISPR/Cas9 on mouse ES cells, HEK cells, hiPSCs and mouse embryos.
  • Cell Culture/Differentiation: ESCs, from hiPSCs to CMs…
  • Super-resolution imaging: STED.
  • Identification of regulatory elements: transient transgenesis assays
  • Generation of transgenic mouse by microinjection or electroporation and consequent embryo transfer.
  • In situ hybridization and inmunofluorescence in whole mount and in paraffin sections
  • Embryo culture.