Leica DM6B Upright Navigator

    Applications

    Wide field fluorescence and transmitted light microscopy, equipped with the Navigator module that allows easy navigation through the sample and acquisition of large images (mosaics).

    SoftwareLeica LAS X 3.4.1.17822
    Objectives

    HC X PL FLUOTAR 5x/0.15

    HC PL Fluotar 10x/0.3 Dry

    HCL APO 10x / 0,3 W

    HC PLAN APO 20x /0,7

    HCX PL APO 40x /1.25

    HCX PL APO lambda blue 63x 1,2 water 

    IncubatorIncubator for temperature control
    Stages

    Motorized stage

    Sample Setup

    Standard microscope slides

    Illumination Source

    Hallogen lamp, Xcite fluorescence lamp

    Detectors/CamerasHamamatsu Orca – Flash 4.0
    Configuration

    Upright

    Epi- Filters

    L5: Exc 460-500nm; Dicr505; Em LP 512-542nm (green)

    Y5: Exc 590-650nm; Dicr660; Em 662-738nm (far red)

    OGC: Exc -; Dicr 497-595; Em - (Recommended for looking through eyepieces)

    GmC: Exc -; Dicr 497-595; Em - (Recommended to see red by combining it with the appropriate ex and em wheels)

    FU2: Exc -; Dicr 410; Em 468-552 (Recommended to see blue in combination with the appropriate excitation wheel)

     

    Ex wheel: GFP (Lw_et430124), mCherry (Lw_et 575/30), Fura-387/11

    Em wheel: GFP (Lw_et 525/36), mCherry (Lw_et 632/60), 100%