Leica SP5 confocal

Confocal for in-vivo or fixed samples multi-colour imaging. Tile scan and automatic multi-positioning available. Wizard for FRET, FRAP experiments.

Leica confocal microscope with temperature and CO2 chamber and working desk
ApplicationsConfocal, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation)
SoftwareLAS-AF 2.7.3. build 9723
ObjectivesHCX PL APO CS 10x 0,4 dry
HCX PL APO lambda blue 20x 0,7 multi-immersion
HCX PL APO CS 40x 1,25 oil
HCX PL APO lambda blue 63x 1,2 water
HCX PL APO CS 63 x 1,3 Glyc
HCX PL APO lambda blue 63x 1,40 oil
Also under previous request:
     HC PL FLUOTAR 20x/0.50 PH2
     HCX PL FLUOTAR 5x/0.15
IncubatorLarge size incubator for temperature and CO2 control
StageMotorized stage
Sample Setup35 mm Petri dishes and standard microscope slides
Illumination SourceDiode 405 nm
Argon Ion Laser: 458,476,488, 496, 514 nm
Diode 561 nm
HeNe 594 nm
HeNe 633 nm
Intensilight fluorescence lamp
Detectors/Cameras4 spectral PMT detectors
1 spectral Hybrid Detector
1 PMT for transmitted light
Epi- FiltersDAPI (A): exc BP 340-380 Dichr400 Em LP 425
Green (I3): exc BP 450-490 Dichr510 Em LP 515
Green Restrictive (L5): exc BP 460-500 Dichr505 Em BP 512-542
Red (N2.1): exc BP 515-560 Dichr580 Em LP 590