Leica SP8 Navigator with on-the-fly deconvolution [cofounded by FEDER “Una manera de hacer Europa”]

Confocal microscope for the multidimensional acquisition of samples. With the Lightning and Navigator packages. The first allows the acquisition of higher resolution images by automation of microscope settings for on the fly or subsequent deconvolution of images. The second package allows for easy navigation through samples in diverse sample holders and for the setting and acquisition of complex multidimensional acquisitions.

ApplicationsConfocal and higher-resolution 3D imaging. Lightning and Navigator packages
SoftwareLAS X 3.5.7. 23225
Objectives

HC PL APO CS2 10x/0.4 Dry
HC PL Apo CS2 20x/0.75 IMM
HC PL Apo CS2 40x/1.3 OIL
HC PL Apo CS2 63x/1.4 OIL
HC PL Apo CS2 63x/1.3 Glycerol

HC PL FLUOTAR5x/0.15 Dry (under request)

IncubatorYes
StagesMotorized stage
Sample Setup35 mm Petri dishes, standard microscope slides, culture multiwall plates
Illumination SourceDiode 405 nm, 442 nm
White Light Laser: from 470 to 670 nm up to 8 lines simultaneously
Intensilight fluorescence lamp
 Detectors/Cameras2 PMTs full spectral
3 Hybrid detectors full spectral
1 detector for transmitted light
Configuration Inverted
Epi-FiltersBlue (A): exc BP 340-380 Dichr400 Em LP 425
Green (I3): exc BP 450-490 Dichr510 Em LP 515
Red (N2.1): exc BP 515-560 Dichr580 Em LP 590
ScannersGalvano/Resonant
External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes