Leica gated STED-3X- WLL SP8 [cofounded by FEDER “Una manera de hacer Europa”]

    STED super resolved imaging and co-localization in 3D. Optimal for fixed samples. Gating, photon counting detectors and super-continuum laser (WLL) provide high flexibility and sensitivity also in the confocal mode. Also with the Navigator package for easy navigation through samples in diverse sample holders and for the setting and acquisition of complex multidimensional acquisitions.. 

    ApplicationsConfocal and super-resolution 3D STED imaging, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation). Navigator package.
    SoftwareLAS X 3.5.7. 23225
    Objectives

    HC PL APO 10x/0.40 CS2. Dry

    HC PL Apo CS2 20x/0.75 IMM

    HC PL Apo CS2 40x/1.3 OIL

    HC PL Apo CS2 63x/1.4 OIL

    HC PL Apo CS2 93x/1.3 GLY (Under request)

    HC PL Apo CS2 100x/1.4 OIL

    IncubatorYes
    StagesMotorized stage
    Sample Setup35 mm Petri dishes and standard microscope slides
    Illumination SourceDiode 405 nm
    White Light Laser: from 470 to 670 nm up to 8 lines simultaneously
    STED Depletion Laser lines: 592nm, 660nm, 775
    Intensilight fluorescence lamp
    Detectors/Cameras2 PMTs full spectral
    2 Hybrid detectors full spectral
    1 detector for transmitted light
    ConfigurationInverted
    Epi- FiltersBlue (A): exc BP 340-380 Dichr400 Em LP 425
    Green (I3): exc BP 450-490 Dichr510 Em LP 515
    Red (N2.1): exc BP 515-560 Dichr580 Em LP 590
    ScannersGalvano/Resonant
    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes