Leica Thunder Spinning Disk-TimeLapse

    Inverted fluorescence microscope with all wide-field fluorescence and colour techniques. With online and offline deconvolution routines. With navigator module and incubation system. It also has a spinning disk mode (Cicero) that allows multichannel and 3D images to be acquired much faster than a point-by-point scanning confocal microscope and reduces the time per experiment

    ApplicationsInverted fluorescence microscope with all wide-field fluorescence and colour techniques. With online and offline deconvolution routines. With navigator module and incubation system. It also has a spinning disk mode (Cicero) that allows multichannel and 3D images to be acquired much faster than a point-by-point scanning confocal microscope and reduces the time per experiment.
    SoftwareLas X
    Objectives
    • HC PLFluotar 1.25x/0.04/C3.7
    • HC PL FL 5x/0.15
    • HC PL APO 10x/0.40 CS2
    • HC PL FLUOTAR 10x/0.32
    • HC PL APO 20x/0.75 CS2
    • HC PL APO 40x/0.95 CORR
    IncubatorWith a chamber enclosing the microscope, digital control of temperature and CO2 allowing long term in-vivo experiments
    StagesUltra-precise and ultra-fast motorised stage
    Sample SetupMulti-well plates, 35‘, 60’ and 100" Petri dishes, and microscope slides
    Illumination Source
    Lasers
    • LDI-4 multi-line, solid-state laser illuminator 1000mW output power and includes the laser lines:
    • 405nm 300mW
    • 470nm 1000mW
    • 555nm 1000mW
    • 640nm 400mW
    Leds
    • CoolLED pE800MBLLG, 8 Leds individuales que cubren el espectro de 400-740 nm (DAPI - Cy7)
     Detectors/Cameras
    • Leica K5C CameraSensor CMOS. Pixel size: 2.4x2.4um Color Filter RGB (Bayer) Color depth 3x12bits or 3x8 bits adjustable 2x2, 3x3 binning and 16/9 Modes 4k as well as Full HD Binning Modes Exposure time 1ms-10s Dynamic range 70dB, 3200:1 Saturation Capacity 15000e- Live image at 9 fps at Full Resolution, 24 fps at 5 MP (2x2 binning), 31 fps at 3.2 MP (3x3 binning)
    • Leica K8 Camera sCMOS_CICERO. Pixel: 2048x2048 (4.2 MP) Pixel size 6.5um QE: 95% max at 580nm Cooling: Air cooled Bit depth: 8/11/12/16 bit Fps: up to 95fps Binning: 2x2
    • Leica K8 Camera_sCMOSWF.         Pixel: 2048x2048 (4.2 MP) Pixel size 6.5um QE: 95% max at 580nm Cooling: Air cooled Bit depth: 8/11/12/16 bit Fps: up to 95fps Binning: 2x2
    Configuration Inverted
    Epi-Filters
    • Emissionfilter wheel LED8: Posit1:460/80 Posit2:535/70 Posit3:590/50 Posit4:642/80 Post5:100%
    • CYR71010 multiLED Exc436/28 506/21 578/24 730/40 Dich459 523 598 763 Em473/22 539/24 641/78 810/80
    • DFT51010 multi LED Exc391/32, 479/33 554/24 638/31 Dich415 500 572 660 Em435/30 519/25 594/32 695/58
    • Cube Mult-LED Exc 380/55, 470/30, 557/35. Dich 412, 490, 590 Em 429/33 510/34 625/70