Nikon ECLIPSE Ti-TimeLapse

Wide field Fluorescence microscope ideal for in vivo imaging with multiple sample carrier adaptors and flexible to all type of acquisition.

Inverted wide field Nikon microscope with side mounted camera and LED illumination system
ApplicationsTransmitted light, wide field fluorescence microscopy, in vivo imaging
SoftwareNikon AR 4.13.04 (b925) 64bits
ObjectivesPlan Fluor 4x/0,13 PhL DL
Plan Fluor 10x/0,3 Ph1 DLL
S Plan Fluor ELWD 20x/0,45 Ph1 ADM
Plan Apo λ 20x/0,75
Plan Fluor ELWD 40x/0.6 Ph2 DM
IncubatorMini-incubator for temperature control and CO2
StageMotorized stage
Sample Setup35 mm Petri dishes, standard microscope slides, multiwell plates
Illumination SourceCool-LED pe-4000, Hallogen lamp
Detectors/CamerasOrca ER Hamamatsu CCD
sCMOS Andor Zyla
Epi- FiltersDAPI: Exc 377-398nm; Dicr409LP; Em 417-477nm (Semrock DAPI-1160A)
GFP: Exc 457-487nm; Dicr520LP; Em 502-538nm (Semrock GFP-3035B)
RED: Exc 542-582nm; Dicr593LP; Em 604-644nm (Semrock TxRed-4040B)
Infrared Cy5: Exc 608-648nm;Dicr660LP; Em 672-712nm (Semrock Cy5-4040C)
Quadruple: Exc 381-404/461-488/543-566/626-644nm//Dichroics 414-450/500-530/580-611/660-800nm//Em 414-450/500-530/580-611/661-800nm (Semrock LED-DA/FI/TR/Cy5-A-000)