Leica 4-Color dSTORM (Ground-State Depletion Single Molecule Localization)

    Based on standard immunostaining techniques it is a flexible system that combines TIRF and epifluorescence with single molecule localization imaging achieving a lateral resolution of up to 20 nm.  Customized for N&B application on live cells.

    ApplicationsdSTORM/GSD (Ground-State Depletion), TIRF, N&B, wide field microscopy
    SoftwareLAS X 1.1.0.12420
    ObjectivesHCX PL APO 160x NA 1.43 Oil
    HCX PL APO 20x NA 0.7 Dry
    IncubatorLarge size incubator for temperature and CO2 control
    StagesManual in XY, Motorized stage in Z axis
    Sample Setup35 mm Petri dishes and standard microscope slides
    Illumination SourceHigh power solid state diode lasers:
    405 nm (30mW)
    488 nm (300mW)
    532 nm (500mW)
    642 nm (500mW)
    Intensilight fluorescence lamp
    Detectors/CamerasEMCCD back-illuminated camera ANDOR iXON Ultra DU897
    ConfigurationInverted
    FiltersQGS: Blue, Green, Red, FarRed:
         Exc: 400-410; 483-493; 527-537; 637-647.
         Dichroic: 417; 496; 544; 655.
         Em: 421-477; 497-519; 547-621; 666-732.

    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes