Leica 3-Colour Fast TIRF AM

    TIRF (Total Internal Reflection Microscopy) illumination system for selective visualization of close to substrate structures such as the basal plasma membrane, and studies of cell motility, protein aggregation, vesicle traffic, etc. Resolution in Z direction reaches up to 70 nm.  Customized for N&B application on live cells.

    ApplicationsTIRF, wide field and N&B Microscopy
    SoftwareLAS-AF 2.6.0. build 7266
    ObjectivesHCX PL APO 20x 0,7 dry
    HCX PL APO 40x 1,25 oil
    HCX PL APO 100x 1,46 oil
    IncubatorLarge size incubator for temperature and CO2 control.  Pecon Heatable Stage Insert.
    StagesMotorized
    Sample Setup35mm Petri dishes and standard microscope slides
    Illumination SourceLaser Diodes Lines: 488, 564, 633 nm
    Intensilight fluorescence lamp
    Detectors/CamerasAndor DU 897 EMCCD
    Andor DU 8285 VP EMCCD
    ConfigurationInverted
    FiltersGFP: BP 490/20 (500) BP 525/50
    RFP: BP 552/24 (570) BP 605/65
    Cy5: BP 635/10 (650) BP 720/60
    VBG: BP 490/20 422/44 552/24 (435/505/550) BP 465/45 545/55 610/65
    External Accesshttps://www.cnic.es/en/investigacion/2/1187/interes