Leica gated STED-3X- WLL SP8 [cofounded by FEDER “Una manera de hacer Europa”]

STED super resolved imaging and co-localization in 3D. Optimal for fixed samples. Gating, photon counting detectors and super-continuum laser (WLL) provide high flexibility and sensitivity also in the confocal mode. Also with the Navigator package for easy navigation through samples in diverse sample holders and for the setting and acquisition of complex multidimensional acquisitions.

ApplicationsConfocal and super-resolution 3D STED imaging, photobleaching techniques (FRAP, FLIP, FRET, photoconversion and photoactivation). Navigator package.
SoftwareLAS X 3.5.7. 23225

HC PL Fluotar 10x/0.3 Dry (Under request)

HC PL Apo CS2 20x/0.75 IMM

HC PL Apo CS2 40x/1.3 OIL

HC PL Apo CS2 63x/1.4 OIL

HC PL Apo CS2 93x/1.3 GLY

HC PL Apo CS2 100x/1.4 OIL

StagesMotorized stage
Sample Setup35 mm Petri dishes and standard microscope slides
Illumination SourceDiode 405 nm
White Light Laser: from 470 to 670 nm up to 8 lines simultaneously
STED Depletion Laser lines: 592nm, 660nm, 775
Intensilight fluorescence lamp
Detectors/Cameras2 PMTs full spectral
2 Hybrid detectors full spectral
1 detector for transmitted light
Epi- FiltersBlue (A): exc BP 340-380 Dichr400 Em LP 425
Green (I3): exc BP 450-490 Dichr510 Em LP 515
Red (N2.1): exc BP 515-560 Dichr580 Em LP 590