Genomics

The Genomics Unit is dedicated to providing high-quality genomics technology and support to the scientific community at the CNIC and beyond. Currently the Unit specializes in the use of second generation sequencing (NGS) technologies for genome analysis by means of Illumina sequencers.

The Unit provides these cutting-edge genomic technologies offering NGS applications including RNA Seq, Low-input RNA Seq, Small RNA-Seq, ChIP Seq, PCR Seq, Exome analysis, Targeted resequencing, etc. On each sequencing project the Unit’s tasks include project consultation, sample quality check, sample library preparation and data generation.

The team continues to improve methods for low-input RNA Seq applications, in which RNA Seq can be performed from very tiny amounts of starting biological material or directly from cells, even at the single-cell level. Researchers from the CNIC Genomics Unit are already using single-cell transcriptome sequencing to study transcriptome variability in heterogeneous cell populations.  The steps of individual cell capture and sample preparation are automated using a microfluidic system, the C1 Single-Cell Auto Prep System from Fluidigm.

NGS experiments conducted by the CNIC Genomics Unit are presented in the following recent articles in high impact-factor journals:

  • I Menendez-Montes et al.  Myocardial VHL-HIF Signaling Controls an Embryonic Metabolic Switch Essential for Cardiac Maturation. Developmental Cell  2016, 39: 724–739.
  • A Latorre-Pellicer et al. Mitochondrial and nuclear DNA matching shapes metabolism and healthy ageing. Nature. 2016, 535:561-5.
  • MacGrogan D et al. Sequential Ligand-Dependent Notch Signaling Activation Regulates Valve Primordium Formation and Morphogenesis. Circ Res. 2016, 118:1480-97
  • Gómez-Del Arco P et al. The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.Cell Metab. 2016, 23:881-92
  • D'Amato G et al. Sequential Notch activation regulates ventricular chamber development. Nat Cell Biol. 2016; 18:7-20.
  • Sánchez-Aguilera A et al. Estrogen Signaling Selectively Induces Apoptosis of Hematopoietic Progenitors and Myeloid Neoplasms without Harming Steady-State Hematopoiesis. Cell Stem Cell. 2014, 15: 791-804
  • Arranz L et al.. Neuropathy of haematopoietic stem niche is essential for myeloproliferative neoplasms. Nature 2014; 512: 78-81
  • Luxán G et al. Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy. Nature Medicine 2013; 19:193-201.

In addition to providing high-quality genomic services, the Unit performs its own research.