Genomics

The Genomics Unit is dedicated to providing high-quality genomics technology and support to the scientific community at the CNIC and beyond. Currently, the Unit specializes in next generation sequencing (NGS) technologies for genome analysis using Illumina sequencers.

Cutting-edge NGS applications provided by the Unit include general RNA Seq, low-input RNA Seq, small RNA-Seq, ChIP Seq, exome analysis, and targeted resequencing. On each sequencing project, the Unit’s tasks include project consultation, sample quality control, sample library preparation, and data generation.

The Genomics group has experience in cohort studies and offers NGS applications involving the sequencing of a high number of samples.

The team continues to improve methods for low-input RNA Seq applications, in which RNA Seq can be performed from tiny amounts of starting biological material or directly from cells, even at the single-cell level. Researchers from the CNIC Genomics Unit are performing single-cell genomics studies using different technologies, including Smart-seq2 from single-sorted cells and the Chromium (10xGenomics) and C1 Single-Cell Auto Prep (Fluidigm) systems.

NGS experiments conducted by the CNIC Genomics Unit are presented in the following articles in high impact-factor journals:

  • Ortiz-Sánchez P et al. Circ. Res. 2019.;125:170-183
  • Tsilingiri K et al. Circulation, 2019. 139:243-255
  • Acín-Pérez R et al. Science Translational Medicine, 2018, 10(434): eaan4935
  • Bartolomé-Izquierdo N et al. Blood 2017; 129: 2408-2419
  • Latorre-Pellicer A et al. Nature 2016; 535:561-5
  • MacGrogan D et al. Circ Res. 2016; 118:1480-97
  • Gómez-Del Arco P et al. Cell Metab. 2016; 23:881-92
  • D'Amato G et al. Nat Cell Biol. 2016; 18:7-20
  • Sánchez-Aguilera A et al. Cell Stem Cell 2014, 15: 791-804
  • Arranz L et al. Nature 2014; 512: 78-81
  • Luxán G et al. Nature Medicine 2013; 19:193-201

The CNIC Genomics Unit has sequenced over 200 whole human genomes.

In addition to providing high-quality genomics services, the Unit collaborates in  research projects. Currently the group participates in the EU Commission project ImPRESS (H2020-MSCA-COFUND-2016-GA754432).